Human IL-33 Quantikine ELISA Kit | hIL-33 QKit
掲載日情報：2016/08/18 現在Webページ番号：110999
R&D Systems製のHuman IL-33を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月06日 19時55分現在]
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Human IL-33 Quantikine ELISA Kit |
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[在庫・価格 ：2024年05月06日 19時55分現在]
Human IL-33 Quantikine ELISA Kit
文献数： 8
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Human Phospho-TOR (S2448) Monoclonal Antibody / APC- and PE-conjugated Mouse Axl Monoclonal Antibodies |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Cell Lysates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
- Sensitivity
- 1.65 pg/mL
- Assay Range
- 6.25 - 400 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-33
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human IL-33 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-33 in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant human IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-33. Results obtained using natural human IL-33 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-33.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 34.3 | 111 | 218 | 39 | 120 | 230 |
| Standard Deviation | 1.45 | 3.33 | 5 | 2.2 | 5.6 | 12.8 |
| CV% | 4.2 | 3 | 2.3 | 5.6 | 4.7 | 5.6 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 34.1 | 112 | 214 | 36.4 | 116 | 224 |
| Standard Deviation | 1.21 | 3.22 | 5.36 | 2.8 | 6.3 | 10.4 |
| CV% | 3.5 | 2.9 | 2.5 | 7.7 | 5.4 | 4.6 |
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Recovery
The recovery of human IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 96 | 88-103 |
| Cell Culture Media (n=4) | 95 | 81-106 |
| EDTA Plasma (n=4) | 86 | 82-92 |
| Heparin Plasma (n=4) | 84 | 80-90 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-33 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay. 
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Additional Infomation
- Molecule Information
- IL-33
- Aliases
- C9orf26; DVS27; NF-HEV
- Entrez Gene IDs
- 90865 (Human); 77125 (Mouse)
- Background
- IL-33
- IL-33 is a proinflammatory protein that may also regulate gene transcription. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines and is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1alpha or IL-1 beta stimulation. Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment. The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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