抗Lamin A + C抗体 | Anti-Lamin A + C antibody

掲載日情報:2020/04/13 現在Webページ番号:35421

GeneTex社の抗Lamin A + C抗体 | Anti-Lamin A + C antibodyです。

本製品は研究用です。研究用以外には使用できません。

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抗Lamin A + C抗体(#GTX101127)のユーザーレビューはこちら。

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Application
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特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年05月16日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-Lamin A/C, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 47
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:lamin A/C,CDCD1,CDDC,CMD1A,CMT2B1,EMD2,FPL,FPLD,FPLD2,HGPS,IDC,LDP1,LFP,LGMD1B,LMN1,LMNC,LMNL1,MADA,PRO1
Genbank No: 4000
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 GeneTex社 オルガネラマーカー抗体
関連記事 GeneTex社における抗体の品質管理
Anti-Lamin A/C, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 47
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:lamin A/C,CDCD1,CDDC,CMD1A,CMT2B1,EMD2,FPL,FPLD,FPLD2,HGPS,IDC,LDP1,LFP,LGMD1B,LMN1,LMNC,LMNL1,MADA,PRO1
Genbank No: 4000
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年05月16日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Lamin A/C, Rabbit-Poly

文献数: 47

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:lamin A/C,CDCD1,CDDC,CMD1A,CMT2B1,EMD2,FPL,FPLD,FPLD2,HGPS,IDC,LDP1,LFP,LGMD1B,LMN1,LMNC,LMNL1,MADA,PRO1
Genbank No: 4000
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 GeneTex社 オルガネラマーカー抗体
関連記事 GeneTex社における抗体の品質管理

Anti-Lamin A/C, Rabbit-Poly

文献数: 47

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:lamin A/C,CDCD1,CDDC,CMD1A,CMT2B1,EMD2,FPL,FPLD,FPLD2,HGPS,IDC,LDP1,LFP,LGMD1B,LMN1,LMNC,LMNL1,MADA,PRO1
Genbank No: 4000
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES


Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Lamin A + C stained by Lamin A + C antibody (GTX101127) diluted at 1:1000.Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Lamin A + C antibody (GTX101127) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments.
IP samples: HeLa whole cell extract
A. 50 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Lamin A + C protein by 4 μg Lamin A + C antibody (GTX101127)
7.5 % SDS-PAGE
The immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody (GTX101127) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Lamin A + C antibody (GTX101127) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX101127 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The ICC/IF analysis of Lamin A + C antibody was published by Singh N and colleagues in the journal Dis Model Mech in 2016 . PMID: 27483354
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IP
ReactivityHuman, Mouse, Rat


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW 74 kDa. ( Note )
Positive Control HeLa , A431 , NIH-3T3 , *K562
Predict Reactivity Bovine, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.73 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the center region of human Lamin A/C. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1
Cellular Localization Nucleus
Background The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]
Database ・ Gene ID: 4000 LMNA
・ UniProt: P02545 LMNA


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REFERENCE

Application ReferenceApplication/Reactivity
Juen-HaurHwang et al. Journal of Functional Foods 2020; 66 : 103832 Hericium erinaceus enhances neurotrophic factors and prevents cochlear cell apoptosis in senescence accelerated mice.Application : WB
Kuo YY et al. Cell Commun Signal 2019; 17 (1) : 100 Caffeic acid phenethyl ester suppresses androgen receptor signaling and stability via inhibition of phosphorylation on Ser81 and Ser213.Application : WB
Reactivity : Human
Takano M et al. Life Sci 2019; 229 : 173-179 Role of peptide transporter 2 and MAPK signaling pathways in the innate immune response induced by bacterial peptides in alveolar epithelial cells.Application : WB
Reactivity : Human
Kuo CC et al. Theranostics 2019; 9 (9) : 2526-2540 Metastatic Colorectal Cancer Rewrites Metabolic Program Through a Glut3-YAP-dependent Signaling Circuit.Application : WB
Reactivity : Human
Yu HI et al. J Pharm Biomed Anal 2018; 160 : 344-350 Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.Application : WB
Reactivity : Human


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