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抗Vimentin抗体 | Anti-Vimentin antibody

掲載日情報：2020/04/13 現在Webページ番号：35201

ジーンテックス /
GeneTex International Corporation
[メーカー略称：GNT]

GeneTex社の抗Vimentin抗体 | Anti-Vimentin antibodyです。

※ 本製品は研究用です。研究用以外には使用できません。

GeneTex社 ホームページでユーザーレビューが閲覧できます


GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
⇒抗Vimentin抗体(#GTX100619)のユーザーレビューはこちら。

Ratingrating star full rating star full rating star full rating star full rating star half( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)rating star full rating star full rating star full rating star full rating star full( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)rating star full rating star full rating star full rating star full rating star empty( Average 4 based on 1 users reviews)

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特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 ：2024年04月29日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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 納期 文献数
Anti-VIM, Rabbit-Poly
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 110
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名：vimentin
Genbank No： 7431
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Cat/Dog/Human/Mouse/Rat 適用 ELISA,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 Neuroscience Antibody for Learning and Forming Episodic Memory
ノックアウト／ノックダウン検証済み抗ビメンチン抗体
使いっきり抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理
Anti-VIM, Rabbit-Poly
 10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 110
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名：vimentin
Genbank No： 7431
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Cat/Dog/Human/Mouse/Rat 適用 ELISA,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 Neuroscience Antibody for Learning and Forming Episodic Memory
ノックアウト／ノックダウン検証済み抗ビメンチン抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 ：2024年04月29日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-VIM, Rabbit-Poly

文献数： 110

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名：vimentin
Genbank No： 7431
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Cat/Dog/Human/Mouse/Rat 適用 ELISA,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 Neuroscience Antibody for Learning and Forming Episodic Memory
ノックアウト／ノックダウン検証済み抗ビメンチン抗体
使いっきり抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理

Anti-VIM, Rabbit-Poly

文献数： 110

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名：vimentin
Genbank No： 7431
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Cat/Dog/Human/Mouse/Rat 適用 ELISA,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 Neuroscience Antibody for Learning and Forming Episodic Memory
ノックアウト／ノックダウン検証済み抗ビメンチン抗体
獣医学研究用抗体 VetSignalシリーズ
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES


Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: Vimentin protein stained by Vimentin antibody (GTX100619) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody (GTX100619) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin antibody detects Vimentin protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Vimentin antibody detects Vimentin protein by western blot analysis.
A. 20 μg 293T whole cell lysate/extract
B. 10 μg 293T whole cell lysate/extract
C. 5 μg 293T whole cell lysate/extract
D. 1 μg 293T whole cell lysate/extract
10% SDS-PAGE
Vimentin antibody (GTX100619) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:20000.
Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin antibody detects Vimentin protein at cell membrane and cytoplasm in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
Vimentin antibody (GTX100619) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:2000.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 μg of Vimentin antibody (GTX100619).
Western blot analysis was performed using Vimentin antibody (GTX100619).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
The WB analysis of Vimentin antibody was published by Diao P and colleagues in the journal J Cell Mol Med in 2019 . PMID: 30950191

The WB analysis of Vimentin antibody was published by Offer S and colleagues in the journal J Exp Clin Cancer Res in 2019 . PMID: 31174567
The WB analysis of Vimentin antibody was published by Diao P and colleagues in the journal J Cell Mol Med in 2019 . PMID: 30950191

The WB analysis of Vimentin antibody was published by Diao P and colleagues in the journal J Cell Mol Med in 2019 . PMID: 30950191

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX100619) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
The WB analysis of Vimentin antibody was published by Tsai CH and colleagues in the journal Sci Rep in 2017.PMID: 28195146

The WB analysis of Vimentin antibody was published by Chen JY and colleagues in the journal J Biomed Sci in 2013.PMID: 24359494
Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin antibody detects Vimentin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Vimentin antibody detects Vimentin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Vimentin stained by Vimentin antibody (GTX100619) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC, sELISA
ReactivityHuman, Mouse, Rat


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
ELISA Assay dependent
IHC Assay dependent
sELISA Assay dependent

Note :

sELISA
Capture: GTX629743/GTX629744, Detection: GTX100619/GTX112661
Not tested in other applications.

Calculated MW 54 kDa. ( Note )
Observed MW 56 kDa. The observed M.W. is based on the following publication. PMID: 23185433 and 24926698
Positive Control 293T , HeLa , MDA-MB-231 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , NIH-3T3 , A549*
Predict Reactivity Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 1.39 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms vimentin
Background Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
Database ・ Gene ID: 7431 VIM
・ UniProt: P08670 VIM


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REFERENCE

Application ReferenceApplication/Reactivity
Peng YS et al. Cell Biol Toxicol 2019; (Epub) BSA-bounded p-cresyl sulfate potentiates the malignancy of bladder carcinoma by triggering cell migration and EMT through the ROS/Src/FAK signaling pathway.Application : WB
Reactivity : Human
Chu CA et al. EBioMedicine 2019; 43 : 270-281 MiR-338-5p promotes metastasis of colorectal cancer by inhibition of phosphatidylinositol 3-kinase, catalytic subunit type 3-mediated autophagy pathway.
Chien CH et al. J Biomed Sci 2019; 26 (1) : 77 Enrichment of superoxide dismutase 2 in glioblastoma confers to acquisition of temozolomide resistance that is associated with tumor-initiating cell subsets.Application : WB
Reactivity : Human
Umeh-Garcia M et al. Cancer Res 2019; (Epub) A Novel Bioengineered MicroRNA-127 Prodrug Suppresses The Growth And Metastatic Potential Of Triple Negative Breast Cancer Cells.Application : IHC
Reactivity : Human
Kuo CY et al. Integr Cancer Ther 2019; 18 : 1534735419866924 Ethanol Extracts of Dietary Herb, Alpinia nantoensis, Exhibit Anticancer Potential in Human Breast Cancer Cells.Application : WB
Reactivity : Human


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reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
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