抗BRCA1 [17F8] - ChIP grade抗体 | Anti-BRCA1 [17F8] - ChIP grade antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

	BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.
	Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
	BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.
	Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
	BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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製品情報

Host	Mouse
Clonality	Monoclonal
Clone Name	17F8
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay, IHC
Reactivity	Human, Mouse

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1 μg/ml
IHC-P	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
ChIP assay	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW	220 kDa. ( Note )
Positive Control	293T , A431 , HeLa
Specificity/Sensitivity	This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.

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PROPERTIES

Form	Liquid
Buffer	PBS
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1.55 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Immunogen	Protein fragment expressed in E. coli corresponding to amino acids 762-1315.
Purification	Protein G purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Brca1, Dna Repair Associated , Brcai , Brcc1 , Brovca1 , Fancs , Iris , Pnca4 , Ppp1R53 , Pscp , Rnf53 , Brca1
Cellular Localization	Nuclear/Cytoplasmic
Background	BRCA1-17F8 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the Aexon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
Database	・ Gene ID: 672 ・ UniProt: P38398

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Huang AC et al. Anticancer Res 2019; 39 (4) : 1839-1847 Casticin Induces DNA Damage and Impairs DNA Repair in Human Bladder Cancer TSGH-8301 Cells.	Application : WB Reactivity : Human
Iqbal J et al. PLoS Pathog 2016; 12 (10) : e1005967 histone h2b-ifi16 recognition of nuclear herpesviral genome induces cytoplasmic *-β responses.	Application : WB Reactivity : Human
Alcaraz Silva B et al. DNA Repair (Amst) 2016; 49 : 43108 Differences in the recruitment of DNA repair proteins at subtelomeric and interstitial I-SceI endonuclease-induced DNA double-strand breaks.	Application : IP Reactivity : Human
Johnson BA et al. Virology 2016; 500 : 22-34 The Rb binding domain of HPV31 E7 is required to maintain high levels of DNA repair factors in infected cells.	Application : WB Reactivity : Human
Shang HS et al. Oncol Rep 2016; 36 (4) : 2207-15 Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells.

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