GTX70111 WB Image
BRCA1 antibody [17F8] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70111 WB Image
Non-transfected (?) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade (GTX70111) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70111 ChIP Image
BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay.
The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay.
Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

GTX70111 IP Image
BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay.
6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract.
Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.