抗Histone H2A.XS139ph (phospho Ser139) [GT2311]抗体 | Anti-Histone H2A.XS139ph (phospho Ser139) [GT2311] antibody

掲載日情報:2020/04/13 現在Webページ番号:141525

GeneTex社の抗Histone H2A.XS139ph (phospho Ser139) [GT2311]抗体 | Anti-Histone H2A.XS139ph (phospho Ser139) [GT2311] antibodyです。

本製品は研究用です。研究用以外には使用できません。

特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年05月04日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-Histone H2A.XS139ph (phospho Ser139), Mouse-Mono(GT2311)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 17
説明文
レビューあり
別名:H2A histone family member X,H2A.X,H2A/X,H2AX
クローン:GT2311
Genbank No: 3014
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理
Anti-Histone H2A.XS139ph (pSer139), Phosphorylated, Mouse(GT2311)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 17
説明文
レビューあり
別名:H2A histone family member X,H2A.X,H2A/X,H2AX
クローン:GT2311
Genbank No: 3014
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年05月04日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Histone H2A.XS139ph (phospho Ser139), Mouse-Mono(GT2311)

文献数: 17

説明文 レビューあり
別名:H2A histone family member X,H2A.X,H2A/X,H2AX
クローン:GT2311
Genbank No: 3014
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

Anti-Histone H2A.XS139ph (pSer139), Phosphorylated, Mouse(GT2311)

文献数: 17

説明文 レビューあり
別名:H2A histone family member X,H2A.X,H2A/X,H2AX
クローン:GT2311
Genbank No: 3014
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Mouse
交差性 Human/Mouse/Rat 適用 IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES

Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 μg HCT116 with CPT 30 μM treatment 24 hr whole cell lysate/extract A. 30 μg HCT116 whole with CPT 30 uM treatment cell lysate/extract B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of histone H2A.X (phospho S139) protein by 2 μg histone H2A.X (phospho S139) antibody (GTX628789) 15% SDS-PAGE The immunoprecipitated histone H2A.X (phospho S139) protein was detected by Human histone H2A.X (phospho S139) antibody (GTX628789) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis.
Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min.
Red: H2A.X protein stained by Histone H2A.X (phospho Ser139) antibody (GTX628789) diluted at 1:500.
Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]
Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibody (GTX628789) diluted at 1:500. Blue: Hoechst 33342 staining.

Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract (untreated)
B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr)
15% SDS-PAGE
Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 μg) were separated by 12%-15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract (untreated)
B. 30 μg HCT116 whole cell lysate/extract (30 μM cisplatin treatment for 24hr)
12% SDS-PAGE
Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

The WB analysis of Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] was published by En J and colleagues in the journal PLoS Negl Trop Dis in 2017.PMID: 28783752


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製品情報

HostMouse
ClonalityMonoclonal
Clone NameGT2311
IsotypeIgG1
ApplicationWB, ICC/IF, IHC-P, IP
ReactivityHuman, Mouse, Rat


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW 15 kDa. ( Note )
Positive Control HCT116 (30μM cisplatin treatment for 24hr) , NIH-3T3 (30μM cisplatin treatment for 24hr) , PC-12 (30uM cisplatin treatment for 24h) , U2OS treated UV (50 J/m2)
Predict Reactivity Bovine, Rhesus Monkey(>80% identity)


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PROPERTIES

Form Liquid
Buffer PBS
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.92 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H2A.XS139ph (phospho Ser139). The exact sequence is proprietary.
Purification Affinity purified by Protein G.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms H2A histone family member X , H2A.X , H2A/X , H2AX
Cellular Localization Nucleus
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
Database ・ Gene ID: 3014 H2AFX
・ UniProt: P16104 H2AFX


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REFERENCE

Application ReferenceApplication/Reactivity
Honda T et al. Sci Rep 2020; 10 (1) : 1140 PDZRN3 protects against apoptosis in myoblasts by maintaining cyclin A2 expression.
Honda T et al. Sci Rep 2020; 10 (1) : 1140 PDZRN3 protects against apoptosis in myoblasts by maintaining cyclin A2 expression.Application : ICC/IF
Reactivity : Mouse
Lin ST et al. Front Physiol 2019; 10 : 1308 Transcription Factor Elf3 Modulates Vasopressin-Induced Aquaporin-2 Gene Expression in Kidney Collecting Duct Cells.Application : WB
Reactivity : Mouse
Wang CY et al. Int J Mol Sci 2019; 20 (22) N-Dihydrogalactochitosan Potentiates the Radiosensitivity of Liver Metastatic Tumor Cells Originated from Murine Breast Tumors.Application : WB
Tamanaha-Nakasone A et al. Sci Rep 2019; 9 (1) : 6416 K1 gene transformation activities in AIDS-related and classic type Kaposi's sarcoma: Correlation with clinical presentation.Application : ICC/IF
Reactivity : Human


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