Rat/Mouse Cytochrome c Quantikine ELISA Kit | r/mCytochrome c Qkit
掲載日情報：2016/08/18 現在Webページ番号：111285
R&D Systems製のRat/Mouse Cytochrome cを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月29日 20時15分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Cytochrome c, Mouse/Rat, ELISA Kit, Quantikine M (96 well) |
|
66 | |||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2024年04月29日 20時15分現在]
Cytochrome c, Mouse/Rat, ELISA Kit, Quantikine M (96 well)
文献数： 66
- 商品コード：MCTC0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：0.78 - 25 ng/mL，感度：0.5 ng/mL，測定試料：Cell Lysates (50 uL), Subcellular Fractions (50 uL)，種交差性：Mouse/Rat，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：2.5 hours，測定因子：Cytochrome c 別名：CYCHCS Genbank No： 54205 |
||
|---|---|---|---|
| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
|
||
| 製品記事 |
アポトーシス検出キット特集 ミトコンドリア(Mitochondria)研究用製品特集 Cytochrome C ELISA Kit |
||
| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
||
追加しました。
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 2.5 hours
- Sample Type & Volume Required Per Well
- Cell Lysates (50 µL), Subcellular Fractions (50 µL)
- Sensitivity
- 0.5 ng/mL
- Assay Range
- 0.78 - 25 ng/mL (Cell Lysates, Subcellular Fractions)
- Specificity
- This kit recognizes both the reduced and oxidized forms of natural rat and mouse cytochrome c.
- Interference
- No significant interference observed with available related molecules.
追加しました。
Product Summary
The Quantikine Rat/Mouse Cytochrome c Immunoassay is a 2.5 hour solid phase ELISA designed to measure rat and mouse cytochrome c. It contains natural rat cytochrome c and antibodies raised against cytochrome c, and because of the 100% sequence identity of rat and mouse cytochrome c, this kit can be used to accurately quantitate natural rat or mouse cytochrome c in cell lysates and subcellular fractions.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Lysates, Subcellular Fractions
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.61 | 8.82 | 13.4 | 1.72 | 8.91 | 13.5 |
| Standard Deviation | 0.02 | 0.09 | 0.19 | 0.06 | 0.31 | 0.59 |
| CV% | 1.24 | 1.02 | 1.42 | 3.49 | 3.48 | 4.37 |
追加しました。
Recovery
The recovery of rat/mouse Cytochrome c spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| PBS/0.01% Saponin (n=2) | 94 | 90-98 |
| PBS/0.05% Tween 20 (n=2) | 101 | 100-102 |
| PBS/0.5% Triton X-100 (n=2) | 100 | 96-106 |
| PBS/1% Triton X-100 (n=2) | 98 | 95-101 |
追加しました。
Linearity
To assess the linearity of the assay, five or more samples spiked with various concentrations of rat/mouse Cytochrome c in each matrix were diluted with Calibrator Diluent and then assayed.
追加しました。
Additional Infomation
- Molecule Information
- Cytochrome c
- Aliases
- CYCS
- Entrez Gene IDs
- 54205 (Human)
- Background
- Cytochrome c
- Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Inhibition of store-operated calcium entry attenuates MPP(+)-induced oxidative stress via preservation of mitochondrial function in PC12 cells: involvement of Homer1a.
Chen W, Fei Z, Li X, Liu W, Zhang L
PLoS ONE 2013 8:e83638
Species: Rat
Sample Type: Mitochondria
Rapid detection of an ABT-737-sensitive primed for death state in cells using microplate-based respirometry.
Carey , Gregory , Chen , Hegang, Clerc , Pascalin, Girnun , Geoffrey, Hwang , Hyehyun, Martin , Stuart S, Mehrabian , Zara, Polster , Brian M, Wei , Michael
PLoS ONE 2012 7:e42487
Species: Mouse
Sample Type: Celll Lysates
Antimitochondrial autoantibodies in pemphigus vulgaris: a missing link in disease pathophysiology.
Arredondo J, Chernyavsky AI, Gindi V, Grando SA, Marchenko S
J. Biol. Chem. 2010 285:3695-704
Species: Mouse
Sample Type: Cell Lysates
Augmenter of liver regeneration: an important intracellular survival factor for hepatocytes.
Chaillet JR, Gandhi CR, Harvey SA, Prelich J, Starzl TE, Thirunavukkarasu C, Wang LF, Watkins SC
J. Hepatol. 2008 48:578-88
Species: Mouse
Sample Type: Cell Lysates
Mitochondrially targeted ceramide LCL-30 inhibits colorectal cancer in mice.
Bielawska A, Bielawski J, Clavien PA, Dahm F, Dindo D, Georgiev P, Hannun YA, Jochum W, Nocito A, Szulc ZM
Br. J. Cancer 2007 98:98-105
Species: Mouse
Sample Type: Cell Lysates
Cardiomyocyte-specific Bcl-2 overexpression attenuates ischemia-reperfusion injury, immune response during acute rejection, and graft coronary artery disease.
Balsam LB, Gunawan F, Kaneda H, Kofidis T, Mokhtari GK, Nakae S, Robbins RC, Tanaka M, Terry RD, Tsao PS
Blood 2004 104:3789-96
Species: Mouse
Sample Type: Cell Lysates
Release of intact, monomeric cytochrome c from apoptotic and necrotic cells.
Jemmerson R, LaPlante B, Treeful A
Cell Death Differ. 2002 9:538-48
Species: Mouse
Sample Type: Cell Culture Supernates
Posttranslational N-myristoylation of BID as a molecular switch for targeting mitochondria and apoptosis.
Korsmeyer SJ, Oh KJ, Wei MC, Weiler S, Zha J
Science 2000 290:1761-5
Species: Mouse
Sample Type: Tissue Homogenates
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。