Human Cytochrome c Quantikine ELISA Kit | hCytochrome c QKit
掲載日情報：2016/08/18 現在Webページ番号：111064
R&D Systems製のHuman Cytochrome cを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月29日 20時55分現在]
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Cytochrome c, Human, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2024年04月29日 20時55分現在]
Cytochrome c, Human, ELISA Kit, Quantikine (96 well)
文献数： 29
- 商品コード：DCTC0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.63 - 20 ng/mL，感度：0.31 ng/mL，測定試料：Cell Lysates (20 uL), Subcellular Fractions (100 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC75，測定時間：4.5 hours，測定因子：Cytochrome c 別名：CYCHCS Genbank No： 54205 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 |
アポトーシス検出キット特集 ミトコンドリア(Mitochondria)研究用製品特集 Cytochrome C ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Lysates (20 µL), Subcellular Fractions (100 µL)
- Sensitivity
- 0.31 ng/mL
- Assay Range
- 0.625 - 20 ng/mL (Cell Lysates, Subcellular Fractions)
- Specificity
- Natural human Cytochrome c
- Interference
- Interference observed with other substances.
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Product Summary
The Quantikine Human Cytochrome c Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human cytochrome c in cell lysates.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Lysates, Subcellular Fractions
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 3.64 | 7.31 | 10.7 | 3.54 | 6.74 | 10.2 |
| Standard Deviation | 0.15 | 0.3 | 0.42 | 0.18 | 0.34 | 0.51 |
| CV% | 4.1 | 4.1 | 3.9 | 5.1 | 5 | 5 |
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Linearity
To assess the linearity of the assay, six cell lysates containing high concentrations of Cytochrome c were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Cytochrome c
- Aliases
- CYCS
- Entrez Gene IDs
- 54205 (Human)
- Background
- Cytochrome c
- Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Roux-en-Y gastric bypass reverses renal glomerular but not tubular abnormalities in excessively obese diabetics.
Abumrad NN, Feurer ID, Ikizler A, Isbell JM, Kasim NR, Marks P, Saliba J, Tamboli RA
Surgery 2010 147:282-7
Species: Human
Sample Type: Urine
Antimitochondrial autoantibodies in pemphigus vulgaris: a missing link in disease pathophysiology.
Arredondo J, Chernyavsky AI, Gindi V, Grando SA, Marchenko S
J. Biol. Chem. 2010 285:3695-704
Species: Human
Sample Type: Cell Lysates
Aurora kinase A is a target of Wnt/beta-catenin involved in multiple myeloma disease progression.
Anderson KC, Carlson NE, Carrasco DE, Carrasco DR, Dutta-Simmons J, Gatt M, Gorgun G, Hideshima T, Letai AG, Mani M, Raje N, Tai YT, Takada K, Zhang Y
Blood 2009 114:2699-708
Species: Human
Sample Type: Cell Culture Supernates
Glycyrrhizin protection against 3-morpholinosydnonime-induced mitochondrial dysfunction and cell death in lung epithelial cells.
Han ES, Kim YJ, Lee CS
Life Sci. 2007 80:1759-67
Species: Human
Sample Type: Cell Lysates
Apolipoprotein-J prevention of fetal cardiac myoblast apoptosis induced by ethanol.
Geng YJ, Li Y, Sagar MB, Shelat H, Wassler M
Biochem. Biophys. Res. Commun. 2007 357:157-61
Species: Canine
Sample Type: Cell Lysates
Proton pump inhibitors induce apoptosis of human B-cell tumors through a caspase-independent mechanism involving reactive oxygen species.
De Milito A, Fais S, Federici C, Iessi E, Logozzi M, Lozupone F, Lugini L, Marino ML, Matarrese P, Nilsson A, Perdicchio M, Spada M
Cancer Res. 2007 67:5408-17
Species: Human
Sample Type: Cell Lysates
Mitochondrial-targeted active Akt protects SH-SY5Y neuroblastoma cells from staurosporine-induced apoptotic cell death.
Johnson GV, Jope RS, Mookherjee P, Quintanilla R, Roh MS, Zmijewska AA
J. Cell. Biochem. 2007 102:196-210
Species: Human
Sample Type: Cell Culture Supernates
The apoptosis effect of hispolon from Phellinus linteus (Berkeley & Curtis) Teng on human epidermoid KB cells.
Chen W, He FY, Li YQ
J Ethnopharmacol 2006 105:280-5
Species: Human
Sample Type: Cell Lysates
Hierarchical regulation of mitochondrion-dependent apoptosis by BCL-2 subfamilies.
Cheng EH, Hsieh JJ, Jeffers JR, Kim H, Rafiuddin-Shah M, Tu HC, Zambetti GP
Nat. Cell Biol. 2006 8:1348-58
Species: Human
Sample Type: Cell Culture Supernates
Ebselen inhibits p38 mitogen-activated protein kinase-mediated endothelial cell death by hydrogen peroxide.
Abe S, Ali N, Fujita Y, Izawa Y, Kagami S, Kanematsu Y, Kyaw M, Tamaki T, Tsuchiya K, Yoshizumi M
Eur. J. Pharmacol. 2004 485:127-35
Species: Human
Sample Type: Cell Lysates
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