ヒトインターロイキン37（IL-37）測定キット Human IL-37 ELISA Kit

The cytokine interleukin-1 elicits a wide array of biologic activities that initiate and promote the host response to injury or infection by activating a set of transcription factors, including NFKB and AP1, which in turn induce production of effectors of the inflammatory response. By computational cloning a cDNA encoding IL-1F7 was isolated to some homology to IL-1 originally called FIL1-zeta. The deduced 192-amino acid protein contains a 15- to 30-amino acid prodomain. Although there are multiple splicing variants, IL-1F7b isoform is a 30 kDa protein and shares critical amino acid residues with IL-18. After rigorous activity tests IL-1F7 was officially designated IL-37. IL-37 mRNA has been detected in various hematopoietic organs as well as in other tissues. It has been shown that IL-37 is strongly expressed intracellularly in human monocytes whose expression can be further upregulated by LPS. IL-37 was shown to bind to the IL-18R without eliciting signal transduction. An immunologic capability associated with IL-37 was shown that intratumoral expression of IL-37 by adenovirus induces Th1-dependent anti-tumor immunity. Although IL-37 does not contain a canonical signal peptide at the NH2-terminus, multiple protein species of IL-37 were detected in the culture supernatants with an overexpression of IL-37. One interesting feature IL-37 exhibits is that upon LPS stimulation the NH2-terminal prodomain encompassing 1st-45th amino acid residues is cleaved by caspase-1 and the resulting mature IL-37 actively translocates into the nucleus, suppressing the induction of TNF-α, IL-6, and MIP-2. Interaction of Smad3 with the translocated IL-37 has been an acting mechanism for the IL-37-mediated anti-inflammation, suggesting that IL-37 is a potent inhibitor of innate immunity. Measurement of intracellular IL-37 upon danger signals or proinflammatory cues would provide a novel insight into anti-inflammation.