抗CD34 [MEC14.7]抗体 | Anti-CD34 [MEC14.7] antibody

• 特長
• 価格
• DATA IMAGES
• 製品情報
• APPLICATION
• PROPERTIES
• TARGET
• REFERENCEE(抜粋)

特長

• 高品質の抗体です。
• 幅広い研究分野に関連する抗体を取り揃えています。
• 使用されたアプリケーションや動物種などの情報が充実しています。
• 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

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DATA IMAGES

Staining of mouse H5V cells using CD34 antibody (GTX28158) and purified rat IgG2a isotype control.

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製品情報

Host	Rat
Clonality	Monoclonal
Clone Name	MEC14.7
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, IHC
Reactivity	Human, Mouse, Rabbit

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APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For FACS: Use at a dilution of 1:500. For IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1:500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For IHC-Fr: Use at a dilution of 1:500. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:500. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

41 kDa. ( Note )

Specificity/Sensitivity

Reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The antibody recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries and developing vascular structures in embryonal structures. Furthermore the antibody reacts with hematopoietic progenitors particularly of myelomonocytic colony forming cells.

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PROPERTIES

Form	Liquid
Buffer	Phosphate-buffered saline containing 0.02% sodium azide
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species	Human
Purification	Protein G affinity purified
Conjugation	Unconjugated
Note	For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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TARGET

Synonyms	Cd34 Molecule , Cd34
Cellular Localization	Type I membrane protein.
Background	The antibody is a useful reagent for identification and characterization of capillary endothelial cells. Furthermore the antibody can be used for isolation and characterization of hematopoietic progenitor cells. In summary the antibody can be used for ELISA, immunofluorescence, immunohistochemistry on frozen and paraffin sections, Western blot, immunoprecipitation, flow cytometry and cell separation. Western blot: The reported molecular mass of CD34 is ranging from 100 to 120 kDa, Apart from the HEV form (Sgp90) which is about 90 kDa. MEC14.7 can recognize a band of about 100 kDa from the H5V line but a band of 75 to 85 kDa from normal tissue. Since different patterns of glycosylation of the molecule are seen with different cell types, this might explain the wide range of CD34 molecular mass (from 75-85 kDa to 120 kD).
Database	・ Gene ID: 947 ・ UniProt: P28906

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REFERENCEE(抜粋)

Application Reference	Application/Reactivity
Nishi H et al. J Am Heart Assoc 2019; 8 (20) : e014074 Biodegradable Flow Diverter for the Treatment of Intracranial Aneurysms: A Pilot Study Using a Rabbit Aneurysm Model.	Application : IHC-Fr Reactivity : Rabbit
Yeung TL et al. Clin Cancer Res 2019; Anticancer immunotherapy by MFAP5 blockade inhibits fibrosis and enhances chemosensitivity in ovarian and pancreatic cancer.
Niu L et al. J Biomed Mater Res B Appl Biomater 2018; (Epub) PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion.	Application : IHC-P Reactivity : Rabbit
Chen B et al. J Cell Biochem 2018; (Epub) Characterization of the structure of rabbit anterior cruciate ligament and its stem/progenitor cells.	Application : IHC-P Reactivity : Rabbit
Koon YL et al. Sci Rep 2018; 8 (1) : 9519 Enhanced Delta-Notch Lateral Inhibition Model Incorporating Intracellular Notch Heterogeneity and Tension-Dependent Rate of Delta-Notch Binding that Reproduces Sprouting Angiogenesis Patterns.	Application : ICC/IF Reactivity : Human

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