抗ERK1/2 [p Thr202, p Tyr204]抗体 | Anti-ERK1/2 [p Thr202, p Tyr204] Antibody

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Image

	Western Blot: ERK1/2 [p Thr202, p Tyr204] Antibody [NBP1-19924] - Analysis of p-ERK1/2 (T202/Y204) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate treated with UV(4h) Lane2:Raw264.7 whole cell lysate treated with UV(4h) Lane3:PC12 whole cell lysate treated with UV(4h)
	Immunohistochemistry-Paraffin: ERK1 [p Thr202, p Tyr204] Antibody [NBP1-19924] - Paraffin-embedded human breast carcinoma tissue.
	Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NBP1-19924] - extracts from Jurkat PMA cells.

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Background

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.

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