抗ATG5抗体 | Anti-ATG5 Antibody

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	Western Blot: ATG5 Antibody [NB110-53818] - Total protein from Human HeLa and A431 and Mouse MEF cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
	Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NB110-53818] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ATG5 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Immunohistochemistry: ATG5 Antibody [NB110-53818] - Staining of human liver hepatocytes at 2.5ug/ml. 40X magnification.
	Western Blot: ATG5 Antibody [NB110-53818] - Analysis in mouse wildtype ES cell lysate (Lane 1) using NB110-53818. Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.
	Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NB110-53818] - Staining of SY5Y cells at 1:250. Incubated overnight at 4 degrees. Photo courtesy of an anonymous collaborator.
	Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NB110-53818] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ATG5 [NB110-53818] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Immunohistochemistry: ATG5 Antibody [NB110-53818] - Staining mouse intestine using DAB with hematoxylin counterstain.
	Immunohistochemistry-Paraffin: ATG5 Antibody [NB110-53818] - Staining of mouse knee. Image from verified customer review.
	Simple Western: ATG5 Antibody [NB110-53818] - Simple Western lane view shows a specific band for ATG5 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

ATG5 (autophagy protein 5, also known as APG5-like, apoptosis-specific protein, APG5L, and ASP) belongs to ATG5 family and is an essential protein for autophagy. It is expressed ubiquitously and the expression increases dramatically in apoptotic cells. ATG5 is localized in cytoplasm (can colocalize with nonmuscle actin) and is induced upon apoptotic stimuli. ATG5-ATG12 conjugate forms a complex with several units of ATG16 and this complex inhibits ATG5-TECPR1 direct interactions. After conjugating to ATG12, ATG5 associates with the isolation membrane to form autophagosomes and this conjugation reaction is mediated by ubiquitin E1-like enzyme Atg7 as well as the E2-like enzyme Atg10. The conjugate detaches from the membrane immediately before or after the completion of autophagosome formation. ATG5 play an important role in the apoptotic process, within the modified cytoskeleton, and its expression is a relatively late event in apoptotic process, occurring downstream of caspase activity.

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