抗Histone H3S10ph (phospho Ser10)抗体 | Anti-Histone H3S10ph (phospho Ser10) antibody

掲載日情報:2020/04/13 現在Webページ番号:45512

GeneTex社の抗Histone H3S10ph (phospho Ser10)抗体 | Anti-Histone H3S10ph (phospho Ser10) antibodyです。

本製品は研究用です。研究用以外には使用できません。

特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

目次に戻る

価格

[在庫・価格 :2024年05月04日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数
Anti-Histone H3S10ph (pSer10), Phosphorylated, Rabbit
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 6
説明文
レビューあり
別名:H3/A antibody,H3FA antibody,HIST1H3B antibody,HIST1H3C antibody,HIST1H3D antibody,HIST1H3E antibody,HIST1H3F antibody,HIST1H3G antibody,HIST1H3H antibody,HIST1H3I antibody,HIST1H3J antibody,HIST1H3A antibody,histone H3/j antibody,histone H3/i antibody
Genbank No: 8350
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Bovine/Human/Mouse/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
関連記事 GeneTex社における抗体の品質管理
Anti-Histone H3S10ph (pSer10), Phosphorylated, Rabbit
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 6
説明文
レビューあり
別名:H3/A antibody,H3FA antibody,HIST1H3B antibody,HIST1H3C antibody,HIST1H3D antibody,HIST1H3E antibody,HIST1H3F antibody,HIST1H3G antibody,HIST1H3H antibody,HIST1H3I antibody,HIST1H3J antibody,HIST1H3A antibody,histone H3/j antibody,histone H3/i antibody
Genbank No: 8350
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Bovine/Human/Mouse/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年05月04日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Histone H3S10ph (pSer10), Phosphorylated, Rabbit

文献数: 6

説明文 レビューあり
別名:H3/A antibody,H3FA antibody,HIST1H3B antibody,HIST1H3C antibody,HIST1H3D antibody,HIST1H3E antibody,HIST1H3F antibody,HIST1H3G antibody,HIST1H3H antibody,HIST1H3I antibody,HIST1H3J antibody,HIST1H3A antibody,histone H3/j antibody,histone H3/i antibody
Genbank No: 8350
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Bovine/Human/Mouse/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
関連記事 GeneTex社における抗体の品質管理

Anti-Histone H3S10ph (pSer10), Phosphorylated, Rabbit

文献数: 6

説明文 レビューあり
別名:H3/A antibody,H3FA antibody,HIST1H3B antibody,HIST1H3C antibody,HIST1H3D antibody,HIST1H3E antibody,HIST1H3F antibody,HIST1H3G antibody,HIST1H3H antibody,HIST1H3I antibody,HIST1H3J antibody,HIST1H3A antibody,histone H3/j antibody,histone H3/i antibody
Genbank No: 8350
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Bovine/Human/Mouse/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 抗ヒストン抗体/修飾ヒストン抗体
ゼブラフィッシュ(zebrafish)研究用製品特集
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理



目次に戻る

DATA IMAGES


Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis.
A. 30 μg U2OS whole cell lysate/extract (untreated)
B. 30 μg U2OS whole cell lysate/extract (serum deprivation for 48hr)
C. 30 μg U2OS whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr
15% SDS-PAGE
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.Histone H3S10ph (phospho Ser10) (GTX128116) antibody dilution: 1:100.

Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis.
A. 30 μg 4T1 whole lysate/extract (untreated)
B. 30 μg 4T1 whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
15% SDS-PAGE
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H3S10ph (phospho Ser10) antibody immunoprecipitates HIST1H3B protein in IP experiments.
IP samples: 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr
A. 30 μg 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of HIST1H3B protein by 4 μg Histone H3S10ph (phospho Ser10) antibody (GTX128116)
15 % SDS-PAGE
The immunoprecipitated HIST1H3B protein was detected by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Histone H3 (phospho Ser10) antibody detects HIST1H3A protein at nucleus on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.Histone H3S10ph (phospho Ser10) antibody (GTX128116) dilution: 1:100.
Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 μg of either control rabbit IgG or anti-Histone H3S10ph (phospho Ser10) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.
HIST1H3A antibody histone cluster 1, H3a protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: histone cluster 1, H3a protein stained by HIST1H3A antibody (GTX128116) diluted at 1:500. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody (GTX11304) diluted at 1:500 . Blue: Hoechst 33342 staining.

Untreated (–) and treated (+) HeLa whole cell extracts (5 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:2000.
Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein at chromosome by confocal immunofluorescent analysis.
Sample: Mitotic HeLa cells were fixed in 2% paraformaldehyde for 30 min.
Green: Histone H3 (phospho Ser10) protein stained by Histone H3 (phospho Ser10) antibody (GTX128116) diluted at 1:500.
Red: alpha Tubulin antibody (GTX11304) diluted at 1:2500.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract (untreated)
B. 30 μg Rat2 whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr)
15% SDS-PAGE
Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The ICC/IF analysis of Histone H3S10ph (phospho Ser10) antibody was published by Lin MJ and colleagues in the journal Sci Rep in 2016.PMID: 27819330


目次に戻る

製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay
ReactivityHuman, Mouse, Rat, Zebrafish


目次に戻る

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW 15 kDa. ( Note )
Positive Control HeLa , HeLa (100 ng/ml Nacodazole treatment for 24 hr) , U2OS(100ng/ml Nocodazole treatment for 24hr) , 4T1 (100ng/ml Nocodazole treatment for 24hr) , Rat2 (100 ng/ml Nocodazole treatment for 24 hr)
Predict Reactivity Chimpanzee(>80% identity)


目次に戻る

PROPERTIES

Form Liquid
Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.35 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H3S10ph (phospho Ser10). The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


目次に戻る

TARGET

Cellular Localization Nucleus


目次に戻る

REFERENCE

Application ReferenceApplication/Reactivity
Camacho LE et al. Theriogenology 2018; 116 : 43111 Effects of maternal nutrient restriction followed by realimentation during early and mid-gestation in beef cows. II. Placental development, umbilical blood flow, and uterine blood flow responses to diet-alterations.Application : IHC-P
Reactivity :
Wang WD et al. Int J Mol Sci 2017; 18 (1) Retinoic Acid Protects and Rescues the Development of Zebrafish Embryonic Retinal Photoreceptor Cells from Exposure to Paclobutrazol.Application : IHC-Wm
Reactivity : Zebrafish
Lin MJ et al. Sci Rep 2016; 6 : 36188 Stathmin-like 4 is critical for the maintenance of neural progenitor cells in dorsal midbrain of zebrafish larvae.Application : IHC-Wm
Reactivity : Zebrafish
Santos M et al. Mol Cell Biochem 2015; 399 (43102) : 143-53 LAP1 is a crucial protein for the maintenance of the nuclear envelope structure and cell cycle progression.Application : WB
Reactivity : Human


目次に戻る

お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。