抗Vimentin抗体 | Anti-Vimentin antibody

掲載日情報:2020/04/02 現在Webページ番号:40506

GeneTex社の抗Vimentin抗体 | Anti-Vimentin antibodyです。

本製品は研究用です。研究用以外には使用できません。

特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年05月13日 13時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-VIM, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 23
説明文
KO/KDバリデーション済み抗体。
別名:vimentin
Genbank No: 7431
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,IC,IF,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 ノックアウト/ノックダウン検証済み抗ビメンチン抗体
関連記事 GeneTex社における抗体の品質管理
Anti-VIM, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 23
説明文
KO/KDバリデーション済み抗体。
別名:vimentin
Genbank No: 7431
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human 適用 ELISA,IC,IF,IHC,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 ノックアウト/ノックダウン検証済み抗ビメンチン抗体
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年05月13日 13時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-VIM, Rabbit-Poly

文献数: 23

説明文 KO/KDバリデーション済み抗体。
別名:vimentin
Genbank No: 7431
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human 適用 ELISA,IC,IF,IHC,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 ノックアウト/ノックダウン検証済み抗ビメンチン抗体
関連記事 GeneTex社における抗体の品質管理

Anti-VIM, Rabbit-Poly

文献数: 23

説明文 KO/KDバリデーション済み抗体。
別名:vimentin
Genbank No: 7431
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human 適用 ELISA,IC,IF,IHC,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 ノックアウト/ノックダウン検証済み抗ビメンチン抗体
使いっきり抗体
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES

Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Vimentin protein stained by Vimentin antibody (GTX112661) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.
Vimentin antibody detects Vimentin protein expression by immunohistochemical analysis.Sample:Paraffin-Embedded adult mouse retina. Green: Vimentin protein stained by Vimentin antibody (GTX112661) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Vimentin antibody detects VIM protein by Western blot analysis.A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract10 % SDS-PAGEVimentin antibody (GTX112661) dilution: 1:1000
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.
Vimentin antibody detects VIM protein at cytosol on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. Vimentin antibody (GTX112661) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunofluorescence analysis of methanol-fixed HeLa, using Vimentin(GTX112661) antibody at 1:200 dilution.
Vimentin antibody detects Vimentin protein at cytosol on mouse lymph node by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. Vimentin antibody (GTX112661) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500.
Vimentin antibody detects VIM protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. Vimentin antibody (GTX112661) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (GTX112661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Vimentin antibody detects Vimentin protein at secreted on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Vimentin antibody (GTX112661) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Vimentin antibody detects Vimentin protein at secreted on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Vimentin antibody (GTX112661) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IHC-Fr, ELISA, sELISA
ReactivityHuman, Mouse, Rat


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
ELISA Assay dependent
sELISA Assay dependent

Note :

sELISA
Capture: Capture: GTX629743/GTX629744, Detection: GTX100619/GTX112661

Not tested in other applications.

Calculated MW 54 kDa. ( Note )
Positive Control NT2D1 , 293T , HeLa , HepG2 , Neuro2A , NIH-3T3 , Rat2 , C8D30 , C2C12
Predict Reactivity Zebrafish, Bovine, Dog, Chicken, Guinea pig, Xenopus laevis, Chimpanzee(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 0.95 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms vimentin
Background Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
Database ・ Gene ID: 7431 VIM
・ UniProt: P08670 VIM


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REFERENCEE(抜粋)

Application ReferenceApplication/Reactivity
Tsai CH et al. Biochim Biophys Acta Mol Cell Res 2019; 1867 (2) : 118564 Aza-PBHA, a potent histone deacetylase inhibitor, inhibits human gastric-cancer cell migration via PKC帢-mediated AHR-HDAC interactions.Application : WB
Reactivity : Human
Ho KH et al. Pharmacol Res 2019; 147 : 104390 miR-140 targeting CTSB signaling suppresses the mesenchymal transition and enhances temozolomide cytotoxicity in glioblastoma multiforme.Application : WB
Reactivity : Human
Li JM et al. Ther Adv Med Oncol 2018; 10 : 1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.Application : WB
Reactivity : Human
Terasaki M et al. Anticancer Res 2018; 38 (4) : 2169-2179 Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.Application : WB
Reactivity : Human
Chen CY et al. Int J Mol Sci 2018; 19 (2) Corylin Suppresses Hepatocellular Carcinoma Progression via the Inhibition of Epithelial-Mesenchymal Transition, Mediated by Long Noncoding RNA GAS5.Application : WB
Reactivity : Human


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お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

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