抗HDAC1抗体 | Anti-HDAC1 antibody

掲載日情報:2020/04/13 現在Webページ番号:35142

GeneTex社の抗HDAC1抗体 | Anti-HDAC1 antibodyです。

本製品は研究用です。研究用以外には使用できません。

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GeneTex社では製品を使用したユーザーからのレビューを各国から集め、GeneTex社ホームページで公開しています。各ユーザーの使用アプリケーション、試料の由来動物、希釈倍率、ブロッキング試薬といった実験条件, 実験結果画像など参考となる情報が掲載されています。
抗HDAC1抗体(#GTX100513)のユーザーレビューはこちら。

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Application
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特長

  • 高品質の抗体です。
  • 幅広い研究分野に関連する抗体を取り揃えています。
  • 使用されたアプリケーションや動物種などの情報が充実しています。
  • 多数の論文で使用実績がある信頼性の高い抗体です。

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価格

[在庫・価格 :2024年05月17日 00時01分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
詳細 商品名
  • 商品コード
  • メーカー
  • 包装
  • 価格
  • 在庫
  • 法規制等
納期 文献数
Anti-HDAC1, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 33
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:histone deacetylase 1,GON-10,HD1,KDAC1,RPD3,RPD3L1
Genbank No: 3065
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 GeneTex社 オルガネラマーカー抗体
関連記事 GeneTex社における抗体の品質管理
Anti-HDAC1, Rabbit-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 33
説明文
レビューあり。KO/KDバリデーション済み抗体。
別名:histone deacetylase 1,GON-10,HD1,KDAC1,RPD3,RPD3L1
Genbank No: 3065
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
クロナリティ Polyclonal フォーマット 性状 Purified 吸収処理
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理

[在庫・価格 :2024年05月17日 00時01分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-HDAC1, Rabbit-Poly

文献数: 33

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:histone deacetylase 1,GON-10,HD1,KDAC1,RPD3,RPD3L1
Genbank No: 3065
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 GeneTex社 オルガネラマーカー抗体
関連記事 GeneTex社における抗体の品質管理

Anti-HDAC1, Rabbit-Poly

文献数: 33

説明文 レビューあり。KO/KDバリデーション済み抗体。
別名:histone deacetylase 1,GON-10,HD1,KDAC1,RPD3,RPD3L1
Genbank No: 3065
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse/Rat/Zebrafish 適用 ChIP,IC,IF,IHC,IP,Western Blot
標識 Unlabeled 性状 Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 使いっきり抗体
関連記事 GeneTex社における抗体の品質管理



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DATA IMAGES


Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HDAC1 protein stained by HDAC1 antibody (GTX100513) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HDAC1 antibody detects HDAC1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of HDAC1 antibody (GTX100513) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
HDAC1 antibody (GTX100513) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC1 protein by 2.5 μg of HDAC1 antibody (GTX100513) 10% SDS-PAGE The immunoprecipitated HDAC1 protein was detected by HDAC1 antibody (GTX100513) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1(GTX100513) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1(GTX100513) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HDAC1 antibody (GTX100513) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
HDAC1 antibody (GTX100513) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The WB analysis of HDAC1 antibody was published by Brügger V and colleagues in the journal Nat Commun in 2017.PMID: 28139683
HDAC1 antibody detects HDAC1 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: HDAC1 antibody (GTX100513) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody (GTX100513) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The IHC-Fr analysis of HDAC1 antibody was published by Brügger V and colleagues in the journal Nat Commun in 2017.PMID: 28139683


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製品情報

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ApplicationWB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay
ReactivityHuman, Mouse, Rat


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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW 55 kDa. ( Note )
Positive Control 293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , NIH3T3 , Rat-2 , *E8.5 embryo
Predict Reactivity Bovine, Xenopus laevis(>80% identity)


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PROPERTIES

Form Liquid
Buffer 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration 1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species Human
Immunogen Recombinant protein encompassing a sequence within the center region of human HDAC1. The exact sequence is proprietary.
Purification Purified by antigen-affinity chromatography.
Conjugation Unconjugated
Note For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


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TARGET

Synonyms histone deacetylase 1 , GON-10 , HD1 , KDAC1 , RPD3 , RPD3L1
Cellular Localization Nucleus
Background Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]
Database ・ Gene ID: 3065 HDAC1
・ UniProt: Q13547 HDAC1


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REFERENCE

Application ReferenceApplication/Reactivity
Wu TH et al. Int J Mol Sci 2019; 20 (23) Emetine Synergizes with Cisplatin to Enhance Anti-Cancer Efficacy against Lung Cancer Cells.Application : WB
Reactivity : Human
Lin YC et al. Int J Mol Sci 2019; 20 (7) Nifedipine Modulates Renal Lipogenesis via the AMPK-SREBP Transcriptional Pathway.
2019; (Epub) Loss of ARID1A induces a stemness gene ALDH1A1 expression with histone acetylation in the malignant subtype of cholangiocarcinoma.Application : IP
Yokoji-Takeuchi M et al. Arch Oral Biol 2019; 110 : 104602 A bacterial metabolite induces Nrf2-mediated anti-oxidative responses in gingival epithelial cells by activating the MAPK signaling pathway.Application : WB
Reactivity : Human
Yuanyuan Ye et al. International Journal of Oncology 2019; : 1019-1032 SIP1 serves a role in HBx?induced liver cancer growth and metastasis.Application : WB
Reactivity : Human


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お問い合わせ先

(テクニカルサポート 試薬担当)

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