抗Wnt-7b抗体(Anti-Wnt-7b, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Wnt‑7bLeu32-Glu72 & Thr216-Ala283Accession # P56706
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Wnt-7b in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Wnt-7a is observed and less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rhWnt‑9a, rmWnt-11, and rmWnt-16 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

Western Blot

Detection of Human Wnt‑7b by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Wnt‑7b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt‑7b at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Wingless-type MMTV Integration Site Family, Member 7B
Background	Wnt-7b
background_content	Background: Wnt-7b Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid-modified and are ligands for members of the frizzled family of receptors, which mediates cell-cell communication during development. Human Wnt-7b is synthesized as a 349 amino acid (aa) precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Mature human Wnt-7b shares 99% aa sequence identity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aa sequence identity with Wnt-7a.

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Citations

1. ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling
   Authors: M Vesel, J Rapp, D Feller, E Kiss, L Jaromi, M Meggyes, G Miskei, B Duga, G Smuk, T Laszlo, I Karner, JE Pongracz
   Respir. Res, 2017;18(1):52.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Frozen


2. WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
   Authors: Bruce F Kimler
   J. Endocrinol., 2016;229(2):197-207.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC-P


3. Downregulated miR329 and miR410 promote the proliferation and invasion of oral squamous cell carcinoma by targeting Wnt-7b.
   Authors: Shiah S, Hsiao J, Chang W, Chen Y, Jin Y, Wong T, Huang J, Tsai S, Hsu Y, Chou S, Yen Y, Jiang S, Shieh Y, Chang I, Hsiao M, Chang J
   Cancer Res, 2014;74(24):7560-72.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


4. CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells.
   Authors: Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K
   Int J Oncol, 2012;41(6):2021-8.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury.
   Authors: Volckaert T, Dill E, Campbell A, Tiozzo C, Majka S, Bellusci S, De Langhe SP
   J. Clin. Invest., 2011;121(11):4409-19.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus.
   Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P
   Neuron, 2009;62(4):510-25.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB

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