抗CD45抗体(Anti-CD45, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse CD45Gln24-Lys425Accession # NP_035340
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CD45 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human CD45 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CD45 (Catalog # 114-CD)
Flow Cytometry	2.5 µg/106 cells	Mouse splenocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	Immersion fixed mouse splenocytes

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Related Product & Information

Long Name	Cluster of Differentiation 45
Background	CD45
background_content	Background: CD45 Mouse CD45 (also known as Ly5 and leukocyte common antigen) is a 180‑220 kDa variably glycosylated member of the class 1 subtype of the protein tyrosine phosphatase family. It is synthesized as a 1291 amino acid (aa) precursor that contains a 23 aa signal sequence, a 541 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 705 aa cytoplasmic region. The ECD is coded for by exons 4‑16 of the CD45 gene. Alternate splicing of exon 4 (or A) (aa 30‑74), exon 5 (or B) (aa 75‑123) and exon 6 (or C) (aa 124‑169) define different lymphocyte populations and functional stages. Naïve T cells express exon 5 (CD45 RB), while activated T cells express neither exon 4, 5 or 6 (CD 45 RO). B cells express CD45 RABC, while resting NK cells express CD45 RA. Mouse CD45 ECD shares 60% and 44% aa sequence identity with rat and human full‑length CD45 ECD, respectively.

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Citations

1. Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss
   Authors: MX Hernandez, S Jiang, TA Cole, SH Chu, MI Fonseca, MJ Fang, LA Hohsfield, MD Torres, KN Green, RA Wetsel, A Mortazavi, AJ Tenner
   Mol Neurodegener, 2017;12(1):66.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Metabolic origins of spatial organization in the tumor microenvironment
   Authors: C Carmona-Fo, M Deforet, L Akkari, CB Thompson, JA Joyce, JB Xavier
   Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion
   Authors: J Liu, S Kumar, E Dolzhenko, GF Alvarado, J Guo, C Lu, Y Chen, M Li, MC Dessing, RK Parvez, PE Cippà, AM Krautzberg, G Saribekyan, AD Smith, AP McMahon
   JCI Insight, 2017;2(18):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


4. Immunization with Bacillus Calmette-Gu�rin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain
   Authors: Z Zuo, F Qi, J Yang, X Wang, Y Wu, Y Wen, Q Yuan, J Zou, K Guo, ZB Yao
   Neurobiol. Dis, 2017;101(0):27-39.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis
   Authors: Jong-Gil Park
   Sci Rep, 2016;6(0):27938.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
   Authors: Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A
   PLoS ONE, 2015;10(3):e0119661.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this.
   Authors: Liakhovitskaia A, Rybtsov S, Smith T, Batsivari A, Rybtsova N, Rode C, de Bruijn M, Buchholz F, Gordon-Keylock S, Zhao S, Medvinsky A
   Development, 2014;141(17):3319-23.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


8. Downstream Toll-like receptor signaling mediates adaptor-specific cytokine expression following focal cerebral ischemia.
   J Neuroinflammation, 2012;9(0):174.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. Serine protease inhibition reduces post-ischemic granulocyte recruitment in mouse intestine.
   Authors: Gobbetti T, Cenac N, Motta JP, Rolland C, Martin L, Andrade-Gordon P, Steinhoff M, Barocelli E, Vergnolle N
   Am. J. Pathol., 2012;180(1):141-52.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


10. Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling.
    Authors: Arora D, Stopp S, Bohmer SA, Schons J, Godfrey R, Masson K, Razumovskaya E, Ronnstrand L, Tanzer S, Bauer R, Bohmer FD, Muller JP
    J. Biol. Chem., 2011;286(13):10918-29.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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