抗MIP-1α抗体(Anti-MIP-1α, Mouse-Mono, FITC antibody)

掲載日情報:2018/11/26 現在Webページ番号:31151

MIP-1αに対する抗体(Anti-MIP-1α, Mouse-Mono, FITC )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-MIP-1α, Mouse-Mono(93342), FITC <Anti-CCL3>
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 2
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説明文
別名:CCL3,MIP-1 alpha
クローン:93342
Genbank No: 6348
Protein Accession No: P10147
法規制等
保存条件 4℃,暗所保存 法規備考
抗原種 Human 免疫動物 Mouse クラス IgG 標識 FITC
交差性 Human 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
関連記事

[在庫・価格 :2024年06月11日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-MIP-1α, Mouse-Mono(93342), FITC <Anti-CCL3>

文献数: 2

説明文 別名:CCL3,MIP-1 alpha
クローン:93342
Genbank No: 6348
Protein Accession No: P10147
法規制等
保存条件 4℃,暗所保存 法規備考
抗原種 Human 免疫動物 Mouse
交差性 Human 適用 FCM
標識 FITC 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
関連記事



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Product Details

Species ReactivityHuman
LabelFluorescein
ImmunogenE. coli-derived recombinant human CCL3/MIP-1 alpha Ala27-Ala92Accession # P10147
SourceMonoclonal Mouse IgG2B Clone # 93342
PurificationProtein A or G purified from ascites
SpecificityDetects human CCL3/MIP-1 alpha in Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) CCL4/MIP-1 beta is observed. Does not cross-react with rhCCL1, 2, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 2, 3, 5, 6, 7, CCL9/10MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20.


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Applications and Data

 Recommended
Concentration
Sample
Intracellular Staining by Flow Cytometry10 µL/106 cellsSee below


Intracellular Staining by Flow Cytometry
Detection of CCL3/CCL4 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL3/CCL4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC2701F) or (B) Mouse IgG2B Fluorescein Isotype Control (Catalog # IC0041F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.


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Related Product & Information

Long NameCCL3/MIP-1 alpha and CCL4/MIP-1 beta Cross-reactive
BackgroundCCL3/CCL4
background_contentBackground:
CCL3/CCL4
The Macrophage Inflammatory Proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha. Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Trained immunity in newborn infants of HBV-infected mothers.
    Authors: Hong M, Sandalova E, Low D, Gehring A, Fieni S, Amadei B, Urbani S, Chong Y, Guccione E, Bertoletti A
    Nat Commun, 2015;6(0):6588.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  2. Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive.
    Authors: Hutnick NA, Carnathan D, Demers K, Makedonas G, Ertl HC, Betts MR
    Vaccine, 2010;28(8):1932-41.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow



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