抗YM1抗体(Anti-YM1, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like 3Tyr22-Tyr398Accession # O35744
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse YM1/Chitinase 3-like 3 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	2.5 µg/mL	See below

Western Blot
	Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot. Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse YM1/Chitinase 3‑like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for YM1/Chitinase 3‑like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western
	Detection of Mouse YM1/Chitinase 3‑like 3 by Simple WesternTM. Simple Western lane view shows lysates of mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for YM1/Chitinase 3‑like 3 at approximately 49 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse YM1/Chitinase 3‑like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Entrez Gene IDs	12655 (Mouse)
Background	YM1/Chitinase 3-like 3
background_content	Background: YM1/Chitinase 3-like 3 YM1, also known as Chitinase 3-like 3 and ECF-L, is a secreted 377 amino acid residue lectin that binds heparin and G1cN oligomers. It is produced primarily by macrophages during inflammation. YM1 shares homology with chitinase family proteins but lacks enzymatic activity.

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Citations

1. Dual role of YM1+ M2 macrophages in allergic lung inflammation
   Authors: C Draijer, P Robbe, CE Boorsma, MN Hylkema, BN Melgert
   Sci Rep, 2018;8(1):5105.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


2. TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury
   Proc. Natl. Acad. Sci. U.S.A., 2016;113(50):E8151-E8158.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Severe Reduction in Number and Function of Peripheral T Cells Does Not Afford Protection toward Emphysema and Bronchial Remodeling Induced in Mice by Cigarette Smoke
   Am J Pathol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Diabetic Wounds Exhibit Decreased Ym1 and Arginase Expression with Increased Expression of IL-17 and IL-20
   Authors: Laurie P Shornick
   Adv Wound Care (New Rochelle), 2016;5(11):486-494.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Smoking p66Shc knocked out mice develop respiratory bronchiolitis with fibrosis but not emphysema.
   Authors: Lunghi B, De Cunto G, Cavarra E, Fineschi S, Bartalesi B, Lungarella G, Lucattelli M
   PLoS ONE, 2015;10(3):e0119797.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Not specified


6. Extrathymically generated regulatory T cells control mucosal TH2 inflammation.
   Authors: Josefowicz SZ, Niec RE, Kim HY
   Nature, 2012;482(7385):395-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Lack of IL-4 receptor expression on T helper cells reduces T helper 2 cell polyfunctionality and confers resistance in allergic bronchopulmonary mycosis.
   Authors: Muller U, Piehler D, Stenzel W, Kohler G, Frey O, Held J, Grahnert A, Richter T, Eschke M, Kamradt T, Brombacher F, Alber G
   Mucosal Immunol, 2012;5(3):299-310.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
   Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
   Biomark Insights, 2012;7(0):87-104.
   Species: Mouse
   Sample Type: BALF
   Application: WB


9. Hookworm-induced persistent changes to the immunological environment of the lung.
   Authors: Reece JJ, Siracusa MC, Southard TL, Brayton CF, Urban JF, Scott AL
   Infect. Immun., 2008;76(8):3511-24.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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