抗HNF-3 β抗体(Anti-HNF-3 β, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human HNF‑3 beta /FoxA2Met242-Ser457Accession # Q9Y261
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Chromatin Immunoprecipitation (ChIP)	5 µg/5 x 106 cells	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human HNF‑3 beta /FoxA2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
	Detection of HNF‑3 beta /FoxA2-regulated Genes by Chromatin Immunoprecipitation. Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The E-RABP promoter was detected by standard PCR.
Immunocytochemistry
	HNF‑3 beta /FoxA2 in Endoderm Differentiated BG01V Human Stem Cells. HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

目次に戻る

Related Product & Information

Long Name	Hepatocyte Nuclear Factor-3, beta
Background	HNF-3 beta/FoxA2
background_content	Background: HNF-3 beta/FoxA2 HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

目次に戻る

Citations

1. Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells
   Authors: Y Kondo, T Toyoda, R Ito, M Funato, Y Hosokawa, S Matsui, T Sudo, M Nakamura, C Okada, X Zhuang, A Watanabe, A Ohta, N Inagaki, K Osafune
   Diabetologia, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Generation of thalamic neurons from mouse embryonic stem cells
   Authors: A Shiraishi, K Muguruma, Y Sasai
   Development, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


3. Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS)
   Authors: B Kuebler, B Aran, L Miquel-Ser, Y Muñoz, E Ars, G Bullich, M Furlano, R Torra, M Marti, A Veiga, A Raya
   Stem Cell Res, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines
   Authors: L Miquel-Ser, A Duarri, Y Muñoz, B Kuebler, B Aran, C Costa, M Martí, M Comabella, S Malhotra, X Montalban, A Veiga, A Raya
   Stem Cell Res, 2017;24(0):155-159.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells
   Authors: KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima
   Stem Cell Reports, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells
   Authors: F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier
   Nat Protoc, 2017;12(4):814-827.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
   Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
   Cell Rep, 2017;19(1):20-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Triple Staining Including FOXA2 Identifies Stem Cell Lineages Undergoing Hepatic and Biliary Differentiation in Cirrhotic Human Liver
   Authors: Leslie E Rogler
   J. Histochem. Cytochem., 2016;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: ICC


9. Cell Expansion During Directed Differentiation Of Stem Cells Toward The Hepatic Lineage
   Stem Cells Dev., 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: CyTof


10. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
    Sci Rep, 2016;6(0):31063.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC-Fluorescent

目次に戻る