抗SOX2抗体(Anti-SOX2, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human SOX2Gly135-Met317Accession # P48431
Source	Monoclonal Mouse IgG2A Clone # 245610
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human, mouse, and rat SOX2 in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	4 µg/mL	See below
CyTOF-reported	Zunder, E.R. et al. (2015) Cell Stem Cell 16: 323. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	See below

Immunocytochemistry
	E‑Cadherin and SOX2 in BG01V Human Stem Cells. E‑Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 µg/mL Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E‑Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (green; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot
	Detection of Human, Mouse, and Rat SOX2 by Western Blot. Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	SOX2 in BG01V Human Embryonic Stem Cells. SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
	Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).  View our protocol for Staining Intracellular Molecules.
Simple Western
	Detection of Human SOX2 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background	SOX2
background_content	Background: SOX2 SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).

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Citations

1. Zinc finger proteins orchestrate active gene silencing during embryonic stem cell differentiation
   Authors: S Kwak, TW Kim, BH Kang, JH Kim, JS Lee, HT Lee, IY Hwang, J Shin, JH Lee, EJ Cho, HD Youn
   Nucleic Acids Res., 2018;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation
   Authors: M Naumann, A Pal, A Goswami, X Lojewski, J Japtok, A Vehlow, M Naujock, R Günther, M Jin, N Stanslowsk, P Reinhardt, J Sternecker, M Frickenhau, F Pan-Montoj, E Storkebaum, I Poser, A Freischmid, JH Weishaupt, K Holzmann, D Troost, AC Ludolph, TM Boeckers, S Liebau, S Petri, N Cordes, AA Hyman, F Wegner, SW Grill, J Weis, A Storch, A Hermann
   Nat Commun, 2018;9(1):335.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice
   Authors: O Le, L Palacio, G Bernier, I Batinic-Ha, G Hickson, C Beauséjour
   Stem Cell Reports, 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. TFAP2C regulates transcription in human naive pluripotency by opening enhancers
   Authors: WA Pastor, W Liu, D Chen, J Ho, R Kim, TJ Hunt, A Lukianchik, X Liu, JM Polo, SE Jacobsen, AT Clark
   Nat. Cell Biol., 2018;20(5):553-564.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Generation of the human induced pluripotent stem cell (hiPSC) line PSMi003-A from a patient affected by an autosomal recessive form of Long QT Syndrome type 1
   Authors: M Mura, M Ginevrino, R Zappatore, F Pisano, M Boni, S Castellett, L Crotti, EM Valente, PJ Schwartz, M Gnecchi
   Stem Cell Res, 2018;29(0):170-173.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Generation of the human induced pluripotent stem cell (hiPSC) line PSMi002-A from a patient affected by the Jervell and Lange-Nielsen syndrome and carrier of two compound heterozygous mutations on the KCNQ1 gene
   Authors: M Mura, YK Lee, M Ginevrino, R Zappatore, F Pisano, M Boni, F Dagradi, L Crotti, EM Valente, PJ Schwartz, HF Tse, M Gnecchi
   Stem Cell Res, 2018;29(0):157-161.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
   Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
   Cell Stem Cell, 2018;22(4):543-558.e12.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


8. Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease
   Authors: JY Lee, SH Han, MH Park, B Baek, IS Song, MK Choi, Y Takuwa, H Ryu, SH Kim, X He, EH Schuchman, JS Bae, HK Jin
   Nat Commun, 2018;9(1):1479.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


9. Co-expression of TIMP-1 and its cell surface binding partner CD63 in glioblastomas
   Authors: C Aaberg-Jes, MD Sørensen, ALSA Matos, JM Moreira, N Brünner, A Knudsen, BW Kristensen
   BMC Cancer, 2018;18(1):270.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


10. FM19G11 and Ependymal Progenitor/Stem Cell Combinatory Treatment Enhances Neuronal Preservation and Oligodendrogenesis after Severe Spinal Cord Injury
    Authors: A Alastrue-A, FJ Rodriguez-, EL Mocholi, F De Giorgio, S Erceg, V Moreno-Man
    Int J Mol Sci, 2018;19(1):.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB

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