抗Mouse Sonic Hedgehog/Shh N-Terminus抗体(Anti-Mouse Sonic Hedgehog/Shh N-Terminus antibody)
掲載日情報：2021/01/28 現在Webページ番号：30201
Mouse Sonic Hedgehog/Shh N-Terminusに対する抗体(Anti-Mouse Sonic Hedgehog/Shh N-Terminus )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月04日 17時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Mouse Sonic Hedgehog/Shh N-Terminus MAb (Clone 171018) |
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Anti-Mouse Sonic Hedgehog/Shh N-Terminus MAb (Clone 171018) |
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Anti-Mouse Sonic Hedgehog/Shh N-Terminus MAb (Clone 171018) |
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[在庫・価格 ：2024年05月04日 17時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Mouse Sonic Hedgehog/Shh N-Terminus MAb (Clone 171018)
文献数： 0
- 商品コード：MAB4641-100
- メーカー：RSD
- 包装：100μg
- 価格：¥100,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：HHG1 クローン：171018 Genbank No： 6469 Protein Accession No： Q62226 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,IHC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse Sonic Hedgehog/Shh N-Terminus MAb (Clone 171018)
文献数： 0
- 商品コード：MAB4641-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Mouse Sonic Hedgehog サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAM4642，スタンダードとして#461-SH-025を用いる。 別名：HHG1 クローン：171018 Genbank No： 6469 Protein Accession No： Q62226 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,IHC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
R&D Systems社 Hedgehog Family関連製品 |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse Sonic Hedgehog/Shh N-Terminus MAb (Clone 171018)
文献数： 0
- 商品コード：MAB4641-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：HHG1 クローン：171018 Genbank No： 6469 Protein Accession No： Q62226 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,IHC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
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| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant mouse Sonic HedgehogCys25-Gly198 (Lys122Arg)Accession # Q62226 |
| Source | Monoclonal Rat IgG2A Clone # 171018 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects mouse Sonic Hedgehog in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Dhh(N‑terminus; aa 23‑198) is observed and no cross-reactivity with rmIhh (N-terminus; aa 66‑240), rmDhh (C‑terminus; aa 199‑396), or rmShh (C‑terminus; aa 199-437) is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 2 µg/mL | See below |
| Immunohistochemistry | 8-25 µg/mL | See below |
| Mouse Sonic Hedgehog Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse Sonic Hedgehog/Shh N-Terminus Antibody (Catalog #MAB4641 ) |
| ELISA Detection (Matched Antibody Pair) | 0.5-2.0 µg/mL | Mouse Sonic Hedgehog/Shh N-Terminus Biotinylated Antibody (Catalog #BAM4642 ) |
| ELISA Standard | Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein (Catalog #461-SH ) | |
| Neutralization | Measured by its ability to neutralize Sonic Hedgehog-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 5 µg/mL Recombinant Mouse Sonic Hedgehog N‑Terminus. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization Sonic Hedgehog/Shh Antibody (171018) [Unconjugated] - N-Terminus](http://resources.rndsystems.com/images/datasheets/antibody/Sonic_Hedgehog_MAB4641_Block_Neutralize_9203.jpg) click image to view larger | Alkaline Phosphatase Production Induced by Sonic Hedgehog and Neutralization by Mouse Sonic Hedgehog Antibody.Recombinant Mouse Sonic Hedgehog N‑Terminus (Catalog # 461-SH or 464-SH) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse Sonic Hedgehog N‑Terminus (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Sonic Hedgehog N‑Terminus Monoclonal Antibody (Catalog # MAB4641). The ND50 is typically 5-15 µg/mL. |
| Western Blot |
![Western Blot Sonic Hedgehog/Shh Antibody (171018) [Unconjugated] - N-Terminus](http://resources.rndsystems.com/images/datasheets/antibody/Sonic_Hedgehog_MAB4641_Western_Blot_11250.jpg) click image to view larger | Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot.Western blot shows lysates of human brain tissue, human stomach tissue, mouse brain tissue, and mouse stomach tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunohistochemistry |
![Immunohistochemistry Sonic Hedgehog/Shh Antibody (171018) [Unconjugated] - N-Terminus](http://resources.rndsystems.com/images/datasheets/antibody/Sonic_Hedgehog_MAB4641_Immunohistochemistry_17197.jpg) click image to view larger | Sonic Hedgehog/Shh in Mouse Embryo.Sonic Hedgehog/Shh was detected in immersion fixed frozen sections of mouse embryo (11 d.p.c.) using Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: Sonic Hedgehog/Shh | Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened. |
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| Entrez Gene IDs: | 6469 (Human); 20423 (Mouse) | |
| Alternate Names: | HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS | |
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Related Research Areas
| Axon Guidance and Neuron Migration | ||
| EMT Induction | ||
| Hedgehog Family | ||
| Hedgehogs | ||
| Morphogens | ||
| Retinal Stem Cell Markers | ||
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| Neutralization | |
|---|---|
 | Alkaline Phosphatase Production Induced by Sonic Hedgehog and Neutralization by Mouse Sonic Hedgehog Antibody. Recombinant Mouse Sonic Hedgehog N‑Terminus (Catalog # 461-SH or 464-SH) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse Sonic Hedgehog N‑Terminus (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Sonic Hedgehog N‑Terminus Monoclonal Antibody (Catalog # MAB4641). The ND50 is typically 5-15 µg/mL. |
| Western Blot | |
 | Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human brain tissue, human stomach tissue, mouse brain tissue, and mouse stomach tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunohistochemistry | |
 | Sonic Hedgehog/Shh in Mouse Embryo. Sonic Hedgehog/Shh was detected in immersion fixed frozen sections of mouse embryo (11 d.p.c.) using Rat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB4641) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
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Related Product & Information
| Entrez Gene IDs | 6469 (Human); 20423 (Mouse) |
|---|---|
| Background | Sonic Hedgehog/Shh |
| background_content | Background: Sonic Hedgehog/Shh Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
- The acid-secreting parietal cell as an endocrine source of Sonic Hedgehog during gastric repair.
Authors: Engevik A, Feng R, Yang L, Zavros Y
Endocrinology, 2013;154(12):4627-39.
Species: Mouse
Sample Type: In Vivo
Application: Neut - Sonic hedgehog and other soluble factors from differentiating embryoid bodies inhibit pancreas development.
Authors: Mfopou JK, De Groote V, Xu X, Heimberg H, Bouwens L
Stem Cells, 2007;25(5):1156-65.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development
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