抗α-Smooth Muscle Actin抗体(Anti-α-Smooth Muscle Actin, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Peptide corresponding to residues EEEDSTALVC of the N-term of human alpha -smooth muscle actin (1)
Source	Monoclonal Mouse IgG2A Clone # 1A4
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human  alpha -Smooth Muscle Actin.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1-0.25 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	See below

Western Blot
	Detection of Human alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of human heart tissue and iBJ6 human induced pluripotent stem cell line undifferentiated or cardiac differentiated. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot
	Detection of Mouse and Rat alpha ‑Smooth Muscle Actin by Western Blot. Western blot shows lysates of mouse colon tissue, C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and rat colon tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	alpha ‑Smooth Muscle Actin in BG01V Human Embryonic Stem Cells. alpha ‑Smooth Muscle Actin was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiomyocytes using Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	alpha ‑Smooth Muscle Actin in Human Heart. alpha ‑Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	alpha -Smooth Muscle Actin in Human Breast Cancer Tissue. alpha -Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Intracellular Staining by Flow Cytometry
	Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Simple Western
	Detection of Human alpha ‑Smooth Muscle Actin by Simple WesternTM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or differentiated to cardiomyocytes (+), loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Smooth Muscle Actin at approximately 49 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat alpha ‑Smooth Muscle Actin Monoclonal Antibody (Catalog # MAB1420). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background	alpha-Smooth Muscle Actin
background_content	Background: alpha-Smooth Muscle Actin alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

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Citations

1. Implication of molecular vascular smooth muscle cell heterogeneity among arterial beds in arterial calcification
   Authors: O Espitia, M Chatelais, M Steenman, C Charrier, B Maurel, S Georges, R Houlgatte, F Verrecchia, B Ory, F Lamoureux, D Heymann, Y Gouëffic, T Quillard
   PLoS ONE, 2018;13(1):e0191976.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. NOTCH1-dependent nitric oxide signaling deficiency in hypoplastic left heart syndrome revealed through patient-specific phenotypes detected in bioengineered cardiogenesis
   Authors: SC Hrstka, X Li, TJ Nelson,
   Stem Cells, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-?B and MURF1 levels
   Authors: L Zhou, Y Huang, H Xie, X Mei
   Mol Med Rep, 2017;0(0):.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


4. Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors
   Authors: RCB Wong, SS Hung, S Jackson, V Singh, S Khan, HH Liang, LS Kearns, T Nguyen, A Conquest, M Daniszewsk, AW Hewitt, A Pébay
   Stem Cell Res, 2017;22(0):13-15.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis
   Authors: H Kamata, Y Tsukasaki, T Sakai, R Ikebe, J Wang, A Jeffers, J Boren, S Owens, T Suzuki, M Higashihar, S Idell, TA Tucker, M Ikebe
   Sci Rep, 2017;7(1):4556.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. P2X7 receptor antagonism modulates IL-1? and MMP9 in human atherosclerotic vessels
   Authors: M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Castellano, R Chiesa, O Alfieri, C Foglieni
   Sci Rep, 2017;7(1):4872.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


7. Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy
   Authors: RCB Wong, SY Lim, SSC Hung, S Jackson, S Khan, NJ Van Bergen, E De Smit, HH Liang, LS Kearns, L Clarke, DA Mackey, AW Hewitt, IA Trounce, A Pébay
   Aging (Albany NY), 2017;9(4):1341-1350.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. Cancer-associated fibroblasts promote an immunosuppressive microenvironment through the induction and accumulation of protumoral macrophages
   Authors: H Takahashi, K Sakakura, T Kudo, M Toyoda, K Kaira, T Oyama, K Chikamatsu
   Oncotarget, 2017;8(5):8633-8647.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


9. Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane
   Authors: Y Dong, A Kanda, K Noda, W Saito, S Ishida
   Sci Rep, 2017;7(0):44266.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


10. Neuropathogenesis of Zika Virus in a Highly Susceptible Immunocompetent Mouse Model after Antibody Blockade of Type I Interferon
    Authors: DR Smith, B Hollidge, S Daye, X Zeng, C Blancett, K Kuszpit, T Bocan, JW Koehler, S Coyne, T Minogue, T Kenny, X Chi, S Yim, L Miller, C Schmaljohn, S Bavari, JW Golden
    PLoS Negl Trop Dis, 2017;11(1):e0005296.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded

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