抗Cadherin E抗体(Anti-Cadherin E, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human E-CadherinAsp155-Ile707Accession # P12830
Source	Monoclonal Mouse IgG2B Clone # 180224
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human E-Cadherin in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	MCF‑7 human breast cancer cell line stained in buffer containing Ca2+ and Mg2+
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	3-25 µg/mL	See below

Western Blot
	Detection of Human E‑Cadherin by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, BT‑20 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E‑Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	E‑Cadherin in MCF‑7 Human Cell Line. E‑Cadherin was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	E‑Cadherin in Human Stomach. E‑Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	E‑Cadherin in Human Skin. E‑Cadherin was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human E‑Cadherin Monoclonal Antibody (Catalog # MAB18381) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Background

E-Cadherin

background_content

Background: E-Cadherin Epithelial (E)‑Cadherin (ECAD), also known as Cadherin-1, cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each (amino acids 155-697).

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Citations

1. Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
   Authors: Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi
   Stem Cells Dev, 2014;23(18):2225-36.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Fresh


2. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
   Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
   Cell Transplant, 2012;21(6):1321-39.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Fluorescence


3. Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation.
   Authors: Ogawa Y, Kawamura T, Kimura T, Ito M, Blauvelt A, Shimada S
   Blood, 2009;113(21):5157-66.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Generation of pluripotent stem cells from adult human testis.
   Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
   Nature, 2008;456(7220):344-9.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity.
   Authors: Miyazawa J, Mitoro A, Kawashiri S, Chada KK, Imai K
   Cancer Res., 2004;64(6):2024-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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