抗Poly-Histidine抗体(Anti-Poly-Histidine, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Label	Phycoerythrin
Immunogen	His-tagged peptide
Source	Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl-terminus.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged protein were stained with Mouse Anti-His Tag PE‑conjugated Monoclonal Antibody (Catalog # IC050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Long Name	Histidine Tag
Background	His Tag
background_content	Background: His Tag Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

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Citations

1. Blocking IL-2 Signal In Vivo with an IL-2 Antagonist Reduces Tumor Growth through the Control of Regulatory T Cells
   Authors: T Carmenate, Y Ortíz, M Enamorado, K García-Mar, J Avellanet, E Moreno, L Graça, K León
   J. Immunol., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. PTX3 binds MD-2 and promotes TRIF-dependent immune protection in aspergillosis.
   Authors: Bozza S, Campo S, Arseni B, Inforzato A, Ragnar L, Bottazzi B, Mantovani A, Moretti S, Oikonomous V, De Santis R, Carvalho A, Salvatori G, Romani L
   J Immunol, 2014;193(5):2340-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Evolutionarily conserved paired immunoglobulin-like receptor alpha (PILRalpha) domain mediates its interaction with diverse sialylated ligands.
   Authors: Sun Y, Senger K, Baginski TK, Mazloom A, Chinn Y, Pantua H, Hamidzadeh K, Ramani SR, Luis E, Tom I, Sebrell A, Quinones G, Ma Y, Mukhyala K, Sai T, Ding J, Haley B, Shadnia H, Kapadia SB, Gonzalez LC, Hass PE, Zarrin AA
   J. Biol. Chem., 2012;287(19):15837-50.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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