抗AFP抗体(Anti-AFP, Human/Mouse, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP
Source	Monoclonal Mouse IgG1 Clone # 189502
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	See below
Knockout Validated	alpha ‑Fetoprotein/AFP is specifically detected in HepG2 human hepatocellular carcinoma parental cell line but is not detectable in alpha ‑Fetoprotein/AFP knockout HepG2 cell line.

Western Blot
	Detection of alpha ‑Fetoprotein/AFP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line. alpha ‑Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
	Detection of alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Simple Western
	Detection of Human alpha ‑Fetoprotein/AFP by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Knockout Validated
	Western Blot Shows Human alpha ‑Fetoprotein/AFP Specificity by Using Knockout Cell Line. Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and alpha ‑Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Knockout Validated
	alpha -Fetoprotein/AFP Specificity is Shown by Flow Cytometry in Knockout Cell Line. alpha -Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with Mouse Anti-Human alpha -Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the alpha -Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Entrez Gene IDs	174 (Human); 11576 (Mouse); 24177 (Rat)
Background	alpha-Fetoprotein/AFP
background_content	Background: alpha-Fetoprotein/AFP alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.

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Citations

1. Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research.
   Authors: Yagi T, Kosakai A, Ito D, Okada Y, Akamatsu W, Nihei Y, Nabetani A, Ishikawa F, Arai Y, Hirose N, Okano H, Suzuki N
   PLoS ONE, 2102;7(7):e41572.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Generation of a human iPSC line from a patient with congenital glaucoma caused by mutation in CYP1B1 gene
   Authors: A Bolinches-, D Lukovic, AA Castro, M León, K Kamenarova, R Kaneva, P Jendelova, F Blanco-Kel, C Ayuso, M Cortón, S Erceg
   Stem Cell Res, 2018;28(0):96-99.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Generation of D1-1 TALEN isogenic control cell line from Dravet syndrome patient iPSCs using TALEN-mediated editing of the SCN1A gene
   Authors: Y Tanaka, T Sone, N Higurashi, T Sakuma, S Suzuki, M Ishikawa, T Yamamoto, J Mitsui, H Tsuji, H Okano, S Hirose
   Stem Cell Res, 2018;28(0):100-104.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. The oncogene Etv5 promotes MET in somatic reprogramming and orchestrates epiblast/primitive endoderm specification during mESCs differentiation
   Authors: J Zhang, H Cao, J Xie, C Fan, Y Xie, X He, M Liao, S Zhang, H Wang
   Cell Death Dis, 2018;9(2):224.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


5. Generation of a gene-corrected isogenic control iPSC line from cystic fibrosis patient-specific iPSCs homozygous for p.Phe508del mutation mediated by TALENs and ssODN
   Authors: S Merkert, C Bednarski, G Göhring, T Cathomen, U Martin
   Stem Cell Res, 2017;23(0):95-97.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet
   Authors: A Schmidt, TV Woedtke, J Stenzel, T Lindner, S Polei, B Vollmar, S Bekeschus
   Int J Mol Sci, 2017;18(4):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease
   Sci Rep, 2016;6(0):32382.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC - Not specified


8. Generation of Naive Bovine Induced Pluripotent Stem Cells Using PiggyBac Transposition of Doxycycline-Inducible Transcription Factors.
   Authors: Kawaguchi T, Tsukiyama T, Kimura K, Matsuyama S, Minami N, Yamada M, Imai H
   PLoS ONE, 2015;10(8):e0135403.
   Species: Bovine
   Sample Type: Whole Cells
   Application: IHC Not Specified


9. Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes.
   Authors: Takenaka C, Miyajima H, Yoda Y, Imazato H, Yamamoto T, Gomi S, Ohshima Y, Kagawa K, Sasaki T, Kawamata S
   PLoS ONE, 2015;10(6):e0129855.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC - PFA fixed


10. Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models.
    Authors: Nguyen L, Robinton D, Seligson M, Wu L, Li L, Rakheja D, Comerford S, Ramezani S, Sun X, Parikh M, Yang E, Powers J, Shinoda G, Shah S, Hammer R, Daley G, Zhu H
    Cancer Cell, 2014;26(2):248-61.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen

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