抗IL-1 Receptor Antagonist抗体(Anti-IL-1 Receptor Antagonist, Human, Mouse-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：28246
IL-1 Receptor Antagonistに対する抗体(Anti-IL-1 Receptor Antagonist, Human, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Summary
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年06月02日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-IL-1 Receptor Antagonist, Human, Mouse-Mono(10309.211) <Anti-IL-1RA> |
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Anti-Human IL-1ra/IL-1F3 MAb (Clone 10309) |
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[在庫・価格 ：2024年06月02日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-IL-1 Receptor Antagonist, Human, Mouse-Mono(10309.211) <Anti-IL-1RA>
文献数： 10
- 商品コード：MAB280
- メーカー：RSD
- 包装：1mg
- 価格：¥114,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human IL-1ra/IL-1F3 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF280，スタンダードとして#280-RA-010を用いる。 別名：DIRA クローン：10309 Genbank No： 3557 Protein Accession No： P18510 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Mouse | ||||
| 交差性 | Human | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human IL-1ra/IL-1F3 MAb (Clone 10309)
文献数： 1
- 商品コード：MAB280-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：DIRA クローン：10309 Genbank No： 3557 Protein Accession No： P18510 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human IL‑1ra/IL-1F3Arg26-Glu177Accession # P18510 |
| Source | Monoclonal Mouse IgG2A Clone # 10309 |
| Purification | Protein A or G purified from ascites |
| Specificity | Detects human IL‑1ra/IL-1F3 in ELISAs. In ELISAs, this antibody shows less than 0.2% cross-reactivity with recombinant mouse (rm) IL‑1ra and no cross-reactivity with rhIL‑1 alpha, rpIL‑1 alpha, rmIL‑1 alpha, rrIL‑1 alpha, rhIL‑1 beta, rpIL‑1 beta, rmIL‑1 beta, rrIL‑1 beta, rhIL‑1 RI, or rhIL‑1 RII. |
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Applications and Data
| Recommended Concentration | Sample | |
| Human IL-1ra/IL-1F3 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human IL‑1ra/IL‑1F3 Antibody (Catalog #MAB280 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human IL‑1ra/IL‑1F3 Biotinylated Antibody (Catalog #BAF280 ) |
| ELISA Standard | Recombinant Human IL-1ra/IL-1F3 Protein (Catalog #280-RA ) | |
| Neutralization | Measured by its ability to neutralize IL‑1ra/IL‑1F3 inhibition of IL‑1 alpha /IL‑1F1-dependent proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 10-20 µg/mL in the presence of 50 ng/mL Recombinant Human IL‑1ra/IL‑1F3, 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1 and 1.25 µg/mL concanavalin A. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
 click image to view larger | IL‑1ra/IL‑1F3 Inhibition of IL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutralization by Human IL‑1ra/IL‑1F3 Antibody.Recombinant Human IL‑1ra/IL‑1F3 (Catalog # 280-RA) inhibits Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑1 alpha /IL‑1F1 (50 pg/mL) activity elicited by Recombinant Human IL‑1ra/IL‑1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280). The ND50 is typically 10-20 µg/mL in the presence of concanavalin A (1.25 µg/mL). |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-1ra/IL-1F3 | IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia. |
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| Long Name: | Interleukin 1 Receptor Antagonist | |
| Entrez Gene IDs: | 3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine) | |
| Alternate Names: | DIRA; ICIL-1RA; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4 | |
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Related Research Areas
| Cytokine and Growth Factor Receptors on VSMC | ||||||||
| Cytokines and Receptors in Allergy and Asthma | ||||||||
| Cytokines and Receptors in Angiogenesis | ||||||||
| IL-1 Family | ||||||||
| Macrophage Activation Markers | ||||||||
| Neuroinflammation | ||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||
|---|---|---|---|---|---|---|---|---|
| Very Good | Simple Western | Mouse | Anonymous | 04/05/2018 | ||||
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Summary
| Application | Simple Western | |||||||
| Sample Tested | B16-F1 mouse melanoma cell line | |||||||
| Species | Mouse | |||||||
| Excellent | Siteclick antibody biotinylation | Mouse | Anonymous | 10/27/2017 |
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Summary
| Application | Siteclick antibody biotinylation |
| Sample Tested | 3T3-L1 mouse embryonic fibroblast adipose-like cell line |
| Species | Mouse |
| Neutralization | |
|---|---|
 | IL‑1ra/IL‑1F3 Inhibition of IL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutralization by Human IL‑1ra/IL‑1F3 Antibody. Recombinant Human IL‑1ra/IL‑1F3 (Catalog # 280-RA) inhibits Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑1 alpha /IL‑1F1 (50 pg/mL) activity elicited by Recombinant Human IL‑1ra/IL‑1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑1ra/IL‑1F3 Monoclonal Antibody (Catalog # MAB280). The ND50 is typically 10-20 µg/mL in the presence of concanavalin A (1.25 µg/mL). |
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Related Product & Information
| Long Name | Interleukin 1 Receptor Antagonist |
|---|---|
| Entrez Gene IDs | 3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine) |
| Background | IL-1ra/IL-1F3 |
| background_content | Background: IL-1ra/IL-1F3 IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
- Interleukin-36 potently stimulates human M2 macrophages, Langerhans cells and keratinocytes to produce pro-inflammatory cytokines
Authors: Damien Dietrich
Cytokine, 2016;84(0):88-98.
Species: Human
Sample Type: Whole Cells
Application: Neut - Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
J. Immunol., 2010;185(11):7047-56.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development - Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1).
Authors: Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C
Cytokine, 2005;31(2):153-60.
Species: Human
Sample Type: Whole Cells
Application: ICC - trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004;80(6):1521-5.
Species: Human
Sample Type: Plasma
Application: ELISA Development - Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Authors: Copeland S, Siddiqui J, Remick D
J. Immunol. Methods, 2004;284(1):99-106.
Species: Human
Sample Type: Cell Culture Supernates
Application: Array Development
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