抗G-CSF抗体(Anti-G-CSF, Mouse, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：27651
G-CSFに対する抗体(Anti-G-CSF, Mouse, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年06月11日 00時00分現在]
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[在庫・価格 ：2024年06月11日 00時00分現在]
Anti-G-CSF, Mouse, Goat-Poly, Biotin
文献数： 5
- 商品コード：BAF414
- メーカー：RSD
- 包装：50μg
- 価格：¥118,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Mouse G-CSF サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB414-500，スタンダードとして#414-CS-005を用いる。 別名：C17orf33 Genbank No： 1440 Protein Accession No： P09920 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Mouse | 免疫動物 | Goat |
| 交差性 | Mouse | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Biotin |
| Immunogen | E. coli-derived recombinant mouse G-CSF (R&D Systems, Catalog # 414-CS)Val31-Ala208Accession # P09920 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse G-CSF in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) G‑CSF, rhCNTF, recombinant mouse (rm) IL‑6, rmLIF, rmOSM, rmCT‑1, rmLeptin, and rmIL‑11 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse G-CSF (Catalog # 414-CS) |
| Mouse G-CSF Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse G‑CSF Antibody (Catalog #MAB414 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse G‑CSF Biotinylated Antibody (Catalog #BAF414 ) |
| ELISA Standard | Recombinant Mouse G-CSF Protein (Catalog #414-CS ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: G-CSF | G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. |
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| Long Name: | Granulocyte Colony Stimulating Factor | |
| Entrez Gene IDs: | 1440 (Human); 12985 (Mouse) | |
| Alternate Names: | C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin | |
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Related Research Areas
| ALS Related Molecules | ||
| Cytokines and Receptors in Angiogenesis | ||
| Gametes and Fertilization | ||
| IL-6 Family | ||
| Macrophage Activation Markers | ||
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Related Product & Information
| Background | G-CSF |
|---|---|
| background_content | Background: G-CSF G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. |
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Citations
- Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012;58(2):169-77.
Species: Mouse
Sample Type: Plasma
Application: Luminex Assay Development - Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism.
Authors: Cain DW, Snowden PB, Sempowski GD, Kelsoe G
PLoS ONE, 2011;6(5):e19957.
Species: Mouse
Sample Type: Serum
Application: ELISA Development - Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense.
Authors: Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK
J. Exp. Med., 2001;194(4):519-27.
Species: Mouse
Sample Type: BALF
Application: ELISA Development
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