抗G-CSF抗体(Anti-G-CSF, Mouse, Goat-Poly antibody)

掲載日情報:2019/10/21 現在Webページ番号:26988

G-CSFに対する抗体(Anti-G-CSF, Mouse, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-G-CSF, Mouse, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
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説明文
別名:C17orf33
Genbank No: 1440
Protein Accession No: P09920
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Mouse 適用 Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事

[在庫・価格 :2024年06月02日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-G-CSF, Mouse, Goat-Poly

文献数: 1

説明文 別名:C17orf33
Genbank No: 1440
Protein Accession No: P09920
法規制等
保存条件 -20℃ 法規備考
抗原種 Mouse 免疫動物 Goat
交差性 Mouse 適用 Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事



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Product Details

Species ReactivityMouse
LabelUnconjugated
ImmunogenE. coli-derived recombinant mouse G-CSFVal31-Ala208Accession # P09920
SourcePolyclonal Goat IgG
PurificationProtein A or G purified
SpecificityDetects mouse G-CSF in direct ELISAs and Western blots.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLRecombinant Mouse G-CSF (Catalog # 414-CS)
NeutralizationMeasured by its ability to neutralize G‑CSF-induced proliferation in the NFS-60 mouse myeloid cell line. The Neutralization Dose (ND50) is typically <1 µg/mL in the presence of 0.125 ng/mL Recombinant Mouse G‑CSF.


Neutralization
Cell Proliferation Induced by G‑CSF and Neutralization by Goat Anti-Mouse G‑CSF Antibody.
Recombinant Mouse G‑CSF (Catalog # 414-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse G‑CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse G‑CSF Polyclonal Antibody (Catalog # AB-414-NA). The ND50 is typically 1 µg/mL.


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Related Product & Information

Long NameGranulocyte Colony Stimulating Factor
Entrez Gene IDs1440 (Human); 12985 (Mouse)
BackgroundG-CSF
background_contentBackground:
G-CSF
G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Notch-dependent repression of miR-155 in the bone marrow niche regulates hematopoiesis in an NF-kappaB-dependent manner.
    Authors: Wang L, Zhang H, Rodriguez S, Cao L, Parish J, Mumaw C, Zollman A, Kamoka M, Mu J, Chen D, Srour E, Chitteti B, HogenEsch H, Tu X, Bellido T, Boswell H, Manshouri T, Verstovsek S, Yoder M, Kapur R, Cardoso A, Carlesso N
    Cell Stem Cell, 2014;15(1):51-65.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut



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