抗G-CSF抗体(Anti-G-CSF, Human, Goat-Poly antibody)

掲載日情報:2018/11/26 現在Webページ番号:26825

G-CSFに対する抗体(Anti-G-CSF, Human, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-G-CSF, Human, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
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説明文
別名:C17orf33
Genbank No: 1440
Protein Accession No: NP_757373
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 Neutralising,Western Blot
クロナリティ Polyclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事

[在庫・価格 :2024年06月11日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-G-CSF, Human, Goat-Poly

文献数: 1

説明文 別名:C17orf33
Genbank No: 1440
Protein Accession No: NP_757373
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社)
関連記事



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Product Details

Species ReactivityHuman
LabelUnconjugated
ImmunogenE. coli-derived recombinant human G-CSFThr31-Pro204Accession # NP_757373
SourcePolyclonal Goat IgG
PurificationProtein A or G purified
SpecificityDetects human G-CSF in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse G‑CSF is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLSee below
NeutralizationMeasured by its ability to neutralize both natural and recombinant human G‑CSF-induced proliferation in the NFS60 mouse myeloid cell line [Shirafuji, N.et al. (1989) Exp. Hematol. 17:116]. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 0.125 ng/mL Recombinant Human G‑CSF.


Western Blot
Detection of Recombinant Human and Mouse G‑CSF by Western Blot.
Western blot shows 25 ng of Recombinant Human G‑CSF (Catalog # 214-CS) and Recombinant Mouse G‑CSF (Catalog # 414-CS). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human G‑CSF Polyclonal Antibody (Catalog # AB-214-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for G‑CSF at approximately 15-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization
Cell Proliferation Induced by G‑CSF and Neutralization by Human G‑CSF Antibody.
Recombinant Human G‑CSF (Catalog # 214-CS) stimulates proliferation in the NFS60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G‑CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Human G‑CSF Polyclonal Antibody (Catalog # AB-214-NA). The ND50 is typically 0.1-0.3 µg/mL.


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Related Product & Information

Long NameGranulocyte Colony Stimulating Factor
Entrez Gene IDs1440 (Human); 12985 (Mouse)
BackgroundG-CSF
background_contentBackground:
G-CSF
G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
    Authors: Solanilla A, Dechanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
    Blood, 2000;95(12):3758-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut



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