抗BCL2, Mouse-Mono(UMAB225) UltraMAB抗体 | Anti-BCL2, Mouse-Mono(UMAB225) UltraMAB Antibody

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

UltraMAB抗体について
OriGene社製品の中でも、特にUltraMAB抗体シリーズは、ヒト組織の免疫染色(IHC)に適した非常に特異性の高いモノクローナル抗体です。抗原にはTrueMAB抗体と同様、組換え体ヒト全長タンパク質を使用しています。1万種類を超えるタンパク質をスポットしたマイクロアレイを用い、標的タンパク質以外の分子との交差反応がないことを確認済みの、癌研究・病理学・解剖学向けの製品です。

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使用例

	Immunohistochemical staining of paraffin-embedded normal thyroid using mouse anti-BCL2 clone UMAB225 (UM800111) at 1:500 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with EDTA pH 8.0 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Cells exhibit cytoplamic staining .
	Immunohistochemical staining of paraffin-embedded human colon tissue within the normal limits using anti-BCL2 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800117 was diluted 1:500 and detection was done with HRP secondary and DAB chromogen.
	Immunohistochemical staining of paraffin-embedded human thyroid tissue within the normal limits using anti-BCL2 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800117 was diluted 1:500 and detection was done with HRP secondary and DAB chromogen. Thyroid cells show mainly cytoplasmic and membraneous staining.
	Immunohistochemical staining of paraffin-embedded carcinoma of cuman prostate tissue using anti-BCL2 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800117 was diluted 1:500 and detection was done with HRP secondary and DAB chromogen. Tumor cells show mainly cytoplasmic and membraneous staining.
	Immunohistochemical staining of paraffin-embedded human lymph node tissue within the normal limits using anti-BCL2 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800117 was diluted 1:500 and detection was done with HRP secondary and DAB chromogen. Positive cells are mainly cytoplasmic and membraneous staining some nuclear stain..
	Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-BCL2 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800117 was diluted 1:500 and detection was done with HRP secondary and DAB chromogen. Positive cells have nuclear, cytoplasmic, and membraneous stain.
	Immunohistochemical staining of paraffin-embedded human tonsil within the normal limits using anti-BCL2 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. UM800117 was diluted 1:500 and detection was done with HRP secondary and DAB chromogen. Positive cells have nuclear, cytoplasmic, and membraneous stain.
	Immunohistochemical staining of FFPE tonsil with anti-BCL2 UM800117 using heat-induced epitope retrieval HIER at 120C for 3min with EDTA buffer pH8, BCL2 mouse monoclonal antibody, clone UMAB225 was used at 1:500. Strong positive cytoplasmic and membrane staining is shown mainly in the non-germinal center.
	Immunohistochemical staining of paraffin-embedded human breast cancer using mouse anti-BCL2 clone UMAB225 (UM800117) at 1:500 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with EDTA pH 8.0 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.
	Immunohistochemical staining of paraffin-embedded human renal cell carcinoma using mouse anti-BCL2 clone UMAB225 (UM800117) at 1:500 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with EDTA pH 8.0 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.
	Immunohistochemical staining of paraffin-embedded lymphoma using mouse anti-BCL2 clone UMAB225 (UM800117) at 1:500 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with EDTA pH 8.0 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.

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製品情報

Clone Name	UMAB225
Applications	WB, IHC
Recommend Dilution	IHC 1:500,
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human BCL2 (NP_000624) produced in HEK293T cell.
Isotype	IgG2a
Formulation	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from cell culture supernatant by affinity chromatography
Predicted Protein Size	26.1 kDa
Gene Name	Homo sapiens BCL2, apoptosis regulator (BCL2), transcript variant alpha
Background	This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008].
Synonyms	Bcl-2; PPP1R50
Database Link	NP_000624

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