抗NKX3-1, Mouse-Mono(UMAB196) UltraMAB抗体 | Anti-NKX3-1, Mouse-Mono(UMAB196) UltraMAB Antibody

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

UltraMAB抗体について
OriGene社製品の中でも、特にUltraMAB抗体シリーズは、ヒト組織の免疫染色(IHC)に適した非常に特異性の高いモノクローナル抗体です。抗原にはTrueMAB抗体と同様、組換え体ヒト全長タンパク質を使用しています。1万種類を超えるタンパク質をスポットしたマイクロアレイを用い、標的タンパク質以外の分子との交差反応がないことを確認済みの、癌研究・病理学・解剖学向けの製品です。

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使用例

	Western blot analysis of extracts (25ug) from 4 different cell lines by using anti-NKX3-1 monoclonal antibody. (1:500) (1: HEK293; 2: Hela; 3: MCF7; 4: HepG2) Dilution: 1:500
	Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
	Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NKX3-1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800088)(1:200)
	Immunofluorescent staining of HeLa cells using anti-NKX3-1 mouse monoclonal antibody (UM800088, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
	OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NKX3-1 mouse monoclonal antibody (UM800088). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)

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製品情報

Clone Name	UMAB196
Applications	WB, IHC, IF, 10K-CHIP
Recommend Dilution	IHC 1:100~200,
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human NKX3-1 (NP_006158) produced in E.coli.
Isotype	IgG1
Formulation	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from cell culture supernatant by affinity chromatography
Predicted Protein Size	26.2 kDa
Gene Name	Homo sapiens NK3 homeobox 1 (NKX3-1), transcript variant 1
Background	This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012].
Synonyms	BAPX2; NKX3; NKX3.1; NKX3A
Database Link	NP_006158

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