抗CDH1, Mouse-Mono(UMAB184) UltraMAB抗体 | Anti-CDH1, Mouse-Mono(UMAB184) UltraMAB Antibody

• 価格
• 特長
• 使用例
• 製品情報
• 文献

価格

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特長

UltraMAB抗体について
OriGene社製品の中でも、特にUltraMAB抗体シリーズは、ヒト組織の免疫染色(IHC)に適した非常に特異性の高いモノクローナル抗体です。抗原にはTrueMAB抗体と同様、組換え体ヒト全長タンパク質を使用しています。1万種類を超えるタンパク質をスポットしたマイクロアレイを用い、標的タンパク質以外の分子との交差反応がないことを確認済みの、癌研究・病理学・解剖学向けの製品です。

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使用例

	Immunohistochemical staining of paraffin-embedded human small intestine using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows mainly membranous staining and weak cytoplasmic staining.
	Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
	Western blot of human tissue lysates (15ug) from 5 different tissues (1: Liver, 2: Ovary, 3: Thyroid, 4: Colon, 5: Spleen ). Diluation: 1:500.
	Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800076)(1:200)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800076)(1:200)
	Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800076)(1:200)
	Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDH1 Mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800076)(1:200)
	IHC staining of FFPE human breast cancer using anti-CDH1 clone UMAB184 mouse monoclonal antibody at 1:200 and detection with Polink2 Broad HRP DAB. UM800076 requires heat-induced epitope retrieval with Accel pH8.7. The image shows strong membranous and some weak cytoplasmic staining in the tumor cells.
	Immunohistochemical staining of paraffin-embedded human skin using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on epithelial cells.
	Immunohistochemical staining of paraffin-embedded human normal cervix using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic and membraneous staining on squamous epithelial cells of the cervix.
	Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on tumor cells.
	Immunohistochemical staining of paraffin-embedded human endocervical gland using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on epithelial cells of the gland.
	Immunohistochemical staining of paraffin-embedded human endometrium using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining on endometrium.
	Immunohistochemical staining of paraffin-embedded human lung using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Stain shows membraneous staining.
	Immunohistochemical staining of paraffin-embedded human thyroid using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining.
	Immunohistochemical staining of paraffin-embedded human ovarian cancer using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
	Immunohistochemical staining of paraffin-embedded human placenta using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining.
	Immunohistochemical staining of paraffin-embedded human prostate cancer using mouse anti-CDH1 clone UMAB184 (UM800076) at 1:200 with Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval with GBI Accel pH 8.7 buffer using pressure chamber for 3 minutes at 110C is required for optimal staining. Shows membraneous staining of the tumor cells.
	OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CDH1 mouse monoclonal antibody (UM800076). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)

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製品情報

Clone Name	UMAB184
Applications	WB, IHC, IF, FC, 10K-CHIP
Recommend Dilution	IHC 1:100~200,
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	The extracellular domain of human CDH1 (NP_004351) produced in human cells.
Isotype	IgG1
Formulation	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from cell culture supernatant by affinity chromatography
Predicted Protein Size	94.8 kDa
Gene Name	Homo sapiens cadherin 1 (CDH1), transcript variant 1
Background	This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008].
Synonyms	Arc-1; BCDS1; CD324; CDHE; ECAD; LCAM; UVO
Database Link	NP_004351

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文献

Telocinobufagin inhibits the epithelial-mesenchymal transition of breast cancer cells through the phosphoinositide 3-kinase/protein kinase B/extracellular signal-regulated kinase/Snail signaling pathway ,Gao, Y;Shi, L;Cao, Z;Zhu, X;Li, F;Wang, R;Xu, J;Zhong, J;Zhang, B;Lu, S;, Oncol Lett 2018 ,PubMed ID 29725474 [CDH1]
Telocinobufagin inhibits the epithelial‑mesenchymal transition of breast cancer cells through the phosphoinositide 3‑kinase/protein kinase B/extracellular signal‑regulated kinase/Snail signaling pathway ,Gao, Y;Shi, L;Cao, Z;Zhu, X;Li, F;Wang, R;Xu, J;Zhong, J;Zhang, B;Lu, S;, Oncol Lett 2018 ,PubMed ID 29725474 [CDH1]

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