抗ABAT, Mouse-Mono(UMAB179) UltraMAB抗体 | Anti-ABAT, Mouse-Mono(UMAB179) UltraMAB Antibody

• 価格
• 特長
• 使用例
• 製品情報

価格

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特長

UltraMAB抗体について
OriGene社製品の中でも、特にUltraMAB抗体シリーズは、ヒト組織の免疫染色(IHC)に適した非常に特異性の高いモノクローナル抗体です。抗原にはTrueMAB抗体と同様、組換え体ヒト全長タンパク質を使用しています。1万種類を超えるタンパク質をスポットしたマイクロアレイを用い、標的タンパク質以外の分子との交差反応がないことを確認済みの、癌研究・病理学・解剖学向けの製品です。

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使用例

	Immunohistochemical staining of paraffin-embedded human liver using ABAT clone UMAB179, mouse monoclonal antibody at 1:400 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800071 requires heat-induced epitope retrieval with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplasmic and membranous staining of the hepatocytes no staining in the bile duct.
	Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
	Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-ABAT monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500) Dilution: 1:500
	Immunohistochemical staining of paraffin-embedded human kidney tissue using anti-ABAT mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) in pressure chamber/cooker at 110C for 3 min, UM800071@ 1:400 shows kidney tubules with strong granular cytoplasmic staining.
	Immunohistochemical staining of paraffin-embedded carcinoma of human liver tissue using anti-ABAT mouse monoclonal antibody.(Heat-induced epitope retrieval in pressure chamber/cooker at 110C for 3 min, UM800071(1:400). Images shows cancer cells with strong granular cytoplasmic staining.
	Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-ABAT mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) in pressure chamber/cooker at 110C for 3 min, UM800071(1:400). Images shows exocrine granular cells with strong granular cytoplasmic staining.
	Immunohistochemical staining of paraffin-embedded carcinoma of human thyroid tissue using ABAT clone UMAB179, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0 ) in pressure chamber/cooker at 110C for 3 min, UM800071 was diluted 1:1000 using HRP detection and DAB chromogen. Image shows strong cytoplasmic and membranous staining is present in the tumor cells.
	Immunofluorescent staining of HepG2 cells using anti-ABAT mouse monoclonal antibody (UM800071, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
	OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ABAT mouse monoclonal antibody (UM800071). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)

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製品情報

Clone Name	UMAB179
Applications	WB, IHC, IF, 10K-CHIP
Recommend Dilution	IHC 1:100~400,
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 29-323 of human ABAT(NP_065737) produced in E.coli.
Isotype	IgG1
Formulation	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from cell culture supernatant by affinity chromatography
Predicted Protein Size	53.2 kDa
Gene Name	Homo sapiens 4-aminobutyrate aminotransferase (ABAT), transcript variant 1
Background	4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008].
Synonyms	GABA-AT; GABAT; NPD009
Database Link	NP_065737

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