抗CD27 [RM27-3E5]組換え抗体 | Anti-CD27 [RM27-3E5] recombinant antibody
掲載日情報:2019/10/24 現在Webページ番号:219196
Absolute Antibody社の抗CD27 [RM27-3E5]組換え抗体(リコンビナント抗体)( Anti-CD27 [RM27-3E5] recombinant antibody)です。
※本製品は研究用です。研究用以外には使用できません。
追加しました。
価格
[在庫・価格 :2024年06月14日 20時55分現在]
詳細 | 商品名 |
|
文献数 | ||
---|---|---|---|---|---|
Anti-CD27, Mouse-Mono [RM27-3E5], IgG2a, Purified Recombinant Antibody |
|
0 | |||
Anti-CD27, Mouse-Mono [RM27-3E5], IgG2a, Purified Recombinant Antibody |
|
0 | |||
[在庫・価格 :2024年06月14日 20時55分現在]
Anti-CD27, Mouse-Mono [RM27-3E5], IgG2a, Purified Recombinant Antibody
文献数: 0
- 商品コード:Ab00895-2.0
- メーカー:ABA
- 包装:200μg
- 価格:¥148,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
別名:TNFRS7,CD27 antigen,CD27L receptor,T-cell activation antigen CD27,Tumor necrosis factor receptor superfamily member 7 クローン:RM27-3E5 |
||
---|---|---|---|
法規制等 | |||
保存条件 | 法規備考 | ||
抗原種 | 免疫動物 | ||
交差性 | Mouse | 適用 | FCM,IP |
標識 | Unlabeled | 性状 | |
吸収処理 | クラス | IgG | |
クロナリティ | Recombinant | フォーマット | |
掲載カタログ |
|
||
製品記事 | |||
関連記事 |
Anti-CD27, Mouse-Mono [RM27-3E5], IgG2a, Purified Recombinant Antibody
文献数: 0
- 商品コード:Ab00895-2.0-BT
- メーカー:ABA
- 包装:1mg
- 価格:ご照会ください
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
別名:TNFRS7,CD27 antigen,CD27L receptor,T-cell activation antigen CD27,Tumor necrosis factor receptor superfamily member 7 クローン:RM27-3E5 |
||
---|---|---|---|
法規制等 | |||
保存条件 | 法規備考 | ||
抗原種 | 免疫動物 | ||
交差性 | Mouse | 適用 | FCM,IP |
標識 | Unlabeled | 性状 | |
吸収処理 | クラス | IgG | |
クロナリティ | Recombinant | フォーマット | |
掲載カタログ |
|
||
製品記事 | |||
関連記事 |
追加しました。
製品情報
Description | Recombinant monoclonal antibody to CD27. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RM27-3E5. This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
---|---|
Species and Isotype | Mouse IgG2a, kappa |
Clone Number | RM27-3E5 |
UniProt Accession Number of Target Protein | P41272 |
Published Application(s) | Neutralisation; FC; IP |
Published Species Reactivity | Mouse |
Immunogen | This antibody was raised by immunising mouse with mouse CD27-human IgG1 Fc fusion protein. Isolated popliteal lymph node cells were then fused with P3U1 myeloma cells to produce stable hybridomas. |
Specificity | This antibody is specific for the extracellular domain of murine CD27, a TNF-receptor superfamily member. |
Application Notes | This antibody recognises murine CD27, as confirmed by flow-cytometry and immunoprecipitation analysis (Sakanishi & Yagita, 2010), a member of the TNF-receptor superfamily. The interaction between CD27 and its ligand CD70, which is recognised by anti-CD70 antibody TAN 1-7 (Ab00816), is crucial to regulation of the survival and differentiation of T cells, B cells and NK cells. As a result, RM27-3E5 modulates multiple immune cell types. RM27-3E5 does not deplete T cells, NKT or NK cells, but does enhance the proliferation of anti-CD3-stimulated splenic T cells (Sakanishi & Yagita, 2010). In murine models of melanoma, it promotes the persistence of tumour-specific CD8+ T cells within tumours, and reduces levels of PD-1 expression on CD8+ T cells (Roberts et al, 2010). Additionally, anti-CD27 reduces the proportion of FoxP3+ CD4+ T cells in tumours, and enhances the effector activity of IFNγ-secreting, tumour-infiltrating CD8+ T cells and NK cells (Roberts et al, 2010). Finally, anti-CD27 Ab treatment alters murine splenocyte cytokine production, significantly reducing IL-5 and IFN-γ synthesis, and increasing TNF-α production (Sumi et al, 2008). It also significantly suppresses total serum IgE levels, while significantly increasing IgG1 and IgG2a levels. These immune modulatory properties translate into potent anti-tumour effects in mice. Treatment of established murine melanoma with RM27-3E5 resulted in a significant reduction in lung metastases and subcutaneous tumours, through an IFNγ-dependent mechanism mediated by CD8+ T cells and NK cells (Roberts et al, 2010). In murine models of T cell lymphoma, the tumour-specific CTL response induced by RM27-3E5 promoted almost complete regression of tumours, regardless of CD27 expression on tumour cells (Sakanishi & Yagita, 2010). This antibody has been used to identify CD27-expressing cells through flow-cytometry (Koyanagi et al, 2012). |
Antibody First Published in | Sumi et al CD27 and CD70 do not play a critical role in the development of experimental allergic conjunctivitis in mice Immunology Letters, Volume 119, Issues 1–2, 15 August 2008, Pages 91-96 PMID:18579220 |
Note on publication | Describes the original generation of this antibody. |
Purification | Protein A affinity purified |
Supplied in | Standard size: PBS with 0.02% Proclin 300. Bulk size: PBS only. |
Storage Recommendation | Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C |
Concentration | See vial label. |
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。