抗CTLA-4 [9H10]組換え抗体 | Anti-CTLA-4 [9H10] recombinant antibody
掲載日情報:2019/10/24 現在Webページ番号:219191
Absolute Antibody社の抗CTLA-4 [9H10]組換え抗体(リコンビナント抗体)( Anti-CTLA-4 [9H10] recombinant antibody)です。
※本製品は研究用です。研究用以外には使用できません。
追加しました。
価格
[在庫・価格 :2024年06月15日 10時55分現在]
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Anti-CTLA-4, Mouse-Mono [9H10], IgG2a, Fc Silent, Purified Recombinant Antibody |
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Anti-CTLA-4, Mouse-Mono [9H10], IgG2a, Fc Silent, Purified Recombinant Antibody |
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[在庫・価格 :2024年06月15日 10時55分現在]
Anti-CTLA-4, Mouse-Mono [9H10], IgG2a, Fc Silent, Purified Recombinant Antibody
文献数: 0
- 商品コード:Ab00894-2.3
- メーカー:ABA
- 包装:200μg
- 価格:¥148,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
別名:CD152,CTLA4,cytotoxic T-lymphocyte-associated antigen 4,Cytotoxic T-lymphocyte protein 4 クローン:9H10 |
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法規制等 | |||
保存条件 | 法規備考 | ||
抗原種 | 免疫動物 | ||
交差性 | Mouse | 適用 | ELISA,FCM,Western Blot |
標識 | Unlabeled | 性状 | |
吸収処理 | クラス | IgG | |
クロナリティ | Recombinant | フォーマット | |
掲載カタログ |
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製品記事 |
Fc Silent組換え抗体 | Fc Silent recombinant antibody 技術情報:抗体のFcドメインのタンパク質工学的改変 |
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関連記事 |
Anti-CTLA-4, Mouse-Mono [9H10], IgG2a, Fc Silent, Purified Recombinant Antibody
文献数: 0
- 商品コード:Ab00894-2.3-BT
- メーカー:ABA
- 包装:1mg
- 価格:ご照会ください
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
別名:CD152,CTLA4,cytotoxic T-lymphocyte-associated antigen 4,Cytotoxic T-lymphocyte protein 4 クローン:9H10 |
||
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法規制等 | |||
保存条件 | 法規備考 | ||
抗原種 | 免疫動物 | ||
交差性 | Mouse | 適用 | ELISA,FCM,Western Blot |
標識 | Unlabeled | 性状 | |
吸収処理 | クラス | IgG | |
クロナリティ | Recombinant | フォーマット | |
掲載カタログ |
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||
製品記事 |
Fc Silent組換え抗体 | Fc Silent recombinant antibody 技術情報:抗体のFcドメインのタンパク質工学的改変 |
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関連記事 |
追加しました。
製品情報
Description | Recombinant monoclonal antibody to CTLA-4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 9H10. This antibody was created using our proprietary Fc Silent™ engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors. This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG2 format, for improved compatibility with existing reagents, assays and techniques. |
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Species and Isotype | Mouse IgG2a, Fc Silent™, kappa |
Clone Number | 9H10 |
UniProt Accession Number of Target Protein | P09793 |
Published Application(s) | Neutralisation; WB; FC; ELISA; proliferation assays |
Published Species Reactivity | Mouse |
Immunogen | This antibody was raised by immunising Syrian hamsters with Staphylococcus A bacteria coated in CTLA-4, isolating B cells from the immunised hamsters and fusing these with the P3X3.Ag8.653 myeloma line to produce stable hybridomas. |
Specificity | This antibody is specific for murine CTLA-4, an inhibitory receptor that acts as the primary negative regulator of T-cell responses. CTLA-4 is expressed predominantly by activated T cells, with significantly higher levels of expression on CD8+ T cells than CD4+ T cells. |
Application Notes | CTLA-4 is upregulated on T cells following their activation, and acts as a negative regulator of T cell responses; CTLA-4 binds to the B7 molecules CD80 and 86, resulting in the delivery of an inhibitory signal, and consequent downregulation of T cell-mediated immunity. Administration of 9H10 blocks the interaction between CTLA-4 on the T cell surface and CD80 and CD86. This promotes the activation of effector T cells and stimulates the immune response raised against weak antigens, including tumour antigens. While this antibody alone does not enhance T cell proliferation, it does significantly increase T cell proliferation when administered together with anti-CD28 (clone 37.51) (Krummel & Allison, 1995), anti-OX40 and anti-GITR (Houot & Levy, 2009). Blocking CTLA-4 induces T cell anti-tumour immunity in animal models, both by suppressing regulatory T cell activity and directly promoting CD8+ T cell effector function (Peggs et al, 2009). In transgenic murine models of prostate cancer, the use of a CTLA-4 blockade in conjunction with an irradiated tumour cell vaccine stimulates an immune response against primary tumours, and results in a significant reduction in tumour incidence (Hurwitz et al, 2000). Similarly, in murine melanoma models, CTLA-4 blockage, in combination with CD40 stimulation and adenoviral vaccination, can elicit complete regression (Sorensen et al, 2010). In murine models of pancreatic ductal adenocarcinoma, 9H10 has also been shown to induce T cell-dependent tumour regressions (Vonderheide et al, 2015). Priming the T cell response with CD40 mAbs or chemotherapy reversed the resistance to 9H10 and RMP1-14 observed in well-established tumours. Additionally, this antibody has been used to detect CTLA-4 using ELISA (Krummel & Allison, 1995) and to stain CTLA-4-expressing cells (Deeths et al, 1999). |
Antibody First Published in | Krummel & Allison CD28 and CTLA-4 Have Opposing Effects on the Response of T cells to Stimulation J Exp Med. 1995 Aug 1; 182(2): 459–465. PMID:7543139 |
Note on publication | Describes the original generation and characterisation of this antibody. |
Purification | Protein A affinity purified |
Supplied in | Bulk size: PBS only. Standard size: PBS with 0.02% Proclin 300. |
Storage Recommendation | Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C |
Concentration | See vial label. |
追加しました。
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