Mouse Clusterin beta EIA Kit
掲載日情報:2017/06/21 現在Webページ番号:202055
RayBiotech社のMouse Clusterin beta EIA Kitです。
※本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2024年06月02日 00時00分現在]
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Mouse Clusterin beta EIA Kit |
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Mouse Clusterin beta EIA Kit |
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Mouse Clusterin beta EIA Kit |
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[在庫・価格 :2024年06月02日 00時00分現在]
Mouse Clusterin beta EIA Kit
文献数: 0
- 商品コード:EIAM-CLUb-1
- メーカー:RAY
- 包装:1kit
- 価格:¥90,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human, Mouse, Rat,アッセイ法:Competition-based,検出方法:呈色,測定試料:細胞培養上清, 血清,感度:4.1 ng, ml,測定範囲:0.1-1,000 ng, ml 別名:Clu |
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保存条件 | 法規備考 | ||
掲載カタログ |
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関連記事 |
Mouse Clusterin beta EIA Kit
文献数: 0
- 商品コード:EIAM-CLUb-2
- メーカー:RAY
- 包装:2kits
- 価格:¥161,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human, Mouse, Rat,アッセイ法:Competition-based,検出方法:呈色,測定試料:細胞培養上清, 血清,感度:4.1 ng, ml,測定範囲:0.1-1,000 ng, ml 別名:Clu |
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法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
|
||
製品記事 | |||
関連記事 |
Mouse Clusterin beta EIA Kit
文献数: 0
- 商品コード:EIAM-CLUb-5
- メーカー:RAY
- 包装:5kits
- 価格:¥357,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human, Mouse, Rat,アッセイ法:Competition-based,検出方法:呈色,測定試料:細胞培養上清, 血清,感度:4.1 ng, ml,測定範囲:0.1-1,000 ng, ml 別名:Clu |
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法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
|
||
製品記事 | |||
関連記事 |
追加しました。
製品説明
■Introduction
Clusterin (CLU, also known as apolipoprotein J) is a member of the small heat shock protein family and a molecular chaperone associated with the clearance of cellular debris and apoptosis. The secretory isoform of CLU is a heterodimeric, disulfide-linked glycoprotein composed of two subunits of 40 kDa alpha- and beta-chains. CLU is a Golgi chaperone that facilitates the folding of secreted proteins in an ATP-independent way. CLU is widely distributed in many tissues and organs, where it participates in a number of biological processes, including lipid transport, membrane recycling, cell adhesion, programmed cell death, and complement-mediated cell lysis. Through this function, CLU is involved in many diseases related to oxidative stress, including neurodegenerative diseases, cancers, inflammatory diseases, and aging. CLU activity is also involved in infectious diseases, such as hepatitis C. As evident by its key roles in cancer development, CLU can serve as a therapeutic target for fighting tumor growth and chemoresistance.
■Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
■Specificity
This EIA kit is designed to detect human, mouse, and rat Clusterin beta chain.
■Target Protein Information
Synonyms: Clusterin beta chain, Apolipoprotein J alpha, ApoJalpha, Complement cytolysis inhibitor a chain
Accession Number: Q06890 PRO_0000005536
Gene Names: Clu
Gene ID: 12759
■Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 µl prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl Stop Solution to each well.
- Read plate at 450 nm immediately.
■Storage/Stability
Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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製品詳細
■ Antigen Information
Gene Symbol:
- CLU
■ Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
■ Array/ELISA Format
Species Detected:
- Human
- Mouse
- Rat
Solid Support: 96-well Microplate
Design Principle:
- Competition-based
Quantitative/Semi-Quantitative: Quantitative
Method of Detection: Colorimetric
Compatible Sample Types:
- Serum
- Cell Culture Supernatants
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