PYY, EIA Kit
掲載日情報:2017/06/21 現在Webページ番号:202033
RayBiotech社のPYY, EIA Kitです。
※本製品は研究用です。研究用以外には使用できません。
追加しました。
価格
[在庫・価格 :2024年06月02日 00時00分現在]
詳細 | 商品名 |
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文献数 | ||
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PYY, EIA Kit (96well) |
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2 | |||
Peptide YY, Human, EIA Kit, RayBio (96-Well Strip Microplate Kit) |
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2 | |||
Human PYY EIA Kit |
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2 | |||
[在庫・価格 :2024年06月02日 00時00分現在]
PYY, EIA Kit (96well)
文献数: 2
- 商品コード:EIA-PYY-1
- メーカー:RAY
- 包装:1kit
- 価格:¥90,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human, Mouse, Rat,感度:2.84 pg, ml,測定範囲:0.1-1,000 pg, ml,検出方法:呈色,測定波長:450 nm,アッセイ法:Competition-based,アッセイ数:96 well,測定試料:細胞培養上清, 血清 別名:PYY |
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法規制等 | |||
保存条件 | -20℃ | 法規備考 | |
掲載カタログ |
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製品記事 | |||
関連記事 |
Peptide YY, Human, EIA Kit, RayBio (96-Well Strip Microplate Kit)
文献数: 2
- 商品コード:EIA-PYY-2
- メーカー:RAY
- 包装:2kits
- 価格:¥161,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human, Mouse, Rat,測定試料:細胞培養上清, 血清,アッセイ法:Competition-based,検出方法:呈色,感度:2.84 pg, ml,測定範囲:0.1-1,000 pg, ml 別名:PYY |
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法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
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||
製品記事 | |||
関連記事 |
Human PYY EIA Kit
文献数: 2
- 商品コード:EIA-PYY-5
- メーカー:RAY
- 包装:5kits
- 価格:¥357,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 |
種交差性:Human, Mouse, Rat,アッセイ法:Competition-based,検出方法:呈色,測定試料:細胞培養上清, 血清,感度:2.84 pg, ml,測定範囲:0.1-1,000 pg, ml 別名:PYY |
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法規制等 | |||
保存条件 | 法規備考 | ||
掲載カタログ |
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||
製品記事 | |||
関連記事 |
追加しました。
製品説明
■Introduction
Peptide YY is a 36 amino acid peptide released by cells in the ileum and colon in response to feeding. It is also known as PYY, Peptide Tyrosine Tyrosine, or Pancreatic Peptide YY3-36.
There are two major forms of Peptide YY: PYY1-36 and PYY3-36 which is the most common form of circulating PYY. Peptide YY3-36 (PYY) is a linear polypeptide consisting of 36 amino acids with structural homology to NPY and pancreatic polypeptide. Circulating PYY concentration increases postprandially and decreases by fasting.
PYY exerts its action through NPY receptors, inhibits gastric motility and increases water and electrolyte absorption in the colon. PYY may also suppress pancreatic secretion. It is secreted by the neuroendocrine cells in the ileum and colon in response to a meal, and has been shown to reduce appetite. PYY works by slowing the gastric emptying; hence, it increases efficiency of digestion and nutrient absorption after meal.
PYY has been shown to play an important role in obesity. Animal studies have shown that acute peripheral administration of PYY3-36 inhibits feeding of rodents and primates. Studies on Y2R-knockout mice have revealed that there is no anorectic effect on Y2R-knockot mice (Y2R is the receptor for PYY). These findings indicate that PYY3-36 has anorectic effect which is suggested to be mediated by Y2R. Studies on PYY-knockout mice have shown that they have higher fat mass and lower glucose tolerance when compared to control mice, indicating that PYY also plays very important role in energy homeostasis by balancing the food intake.
Studies have also shown that obese people secrete less PYY than non-obese people. The anorectic effect of PYY represents a possible anti-obesity therapy in the future.
■Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
■Specificity
Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
This kit detects the 1-36 form PYY.
■Target Protein Information
Synonyms: Peptide YY (PYY) (PYY-I) (Peptide tyrosine tyrosine)
Accession Number: P10082
Gene Names: PYY
Gene ID: 5697
■Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 µl prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl Stop Solution to each well.
- Read plate at 450 nm immediately.
■Storage/Stability
Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
追加しました。
製品詳細
■ Antigen Information
Gene Symbol:
- PYY
■ Product Specifications
Size: 1, 2, or 5 x 96-Well Strip Microplate Kit
■ Array/ELISA Format
Species Detected:
- Human
- Mouse
- Rat
Solid Support: 96-well Microplate
Design Principle:
- Competition-based
Quantitative/Semi-Quantitative: Quantitative
Method of Detection: Colorimetric
Compatible Sample Types:
- Serum
- Cell Culture Supernatants
追加しました。
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