BNP, Human, EIA Kit (Brain Natriuretic Peptide, EIA Kit)

RayBiotech社のBNP, Human, EIA Kit (Brain Natriuretic Peptide, EIA Kit)です。
※本製品は研究用です。研究用以外には使用できません。

■Introduction

Brain natriuretic peptide (BNP), (aka B-type natriuretic peptide), is a 32 amino acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of myocytes in the ventricles. BNP was originally identified in extracts of porcine brain, but in humans it is produced mainly in the cardiac ventricles. Its counterpart in rats is a 45 amino acid peptide hormone. At the time of release, a co-secreted 76 amino acid N-terminal fragment (NT-proBNP) is also released with BNP.

BNP binds to and activates NPRA in a similar fashion to atrial natriuretic peptide (ANP) but with 10-fold lower affinity. The biological half-life of BNP, however, is twice as long as that of ANP. Both ANP and BNP have limited ability to bind and activate NPRB.

Physiologic actions of BNP include decrease in systemic vascular resistance and central venous pressure as well as an increase in natriuresis. Thus, the resulting effect of BNP is a decrease in cardiac output and a decrease in blood volume.

Tests showing elevated levels of BNP or NT-proBNP in blood are used as a diagnosis of heart failure and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with poorer outcome.

Both BNP and NT-proBNP have been approved as a marker for acute congestive heart failure (CHF). The plasma concentrations of both BNP are increased in patients with asymptomatic and symptomatic left ventricular dysfunction. There is no level of BNP that perfectly separates patients with and without heart failure.

■BNP EIA/ELISA Test Kit Features

■Specificity

The capture antibody provided in the BNP EIA/ELISA Test Kit recognizes human, mouse and rat Brain Natriuretic Peptide / NPPB.

■Target Protein Information

Synonyms: Natriuretic peptides B (Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 32 (BNP(1-32)) (BNP-32)]

Accession Number: P16860 (PRO_0000001532)

Gene Names: NPPB

Gene ID: 4879

■Application Notes

Other Materials Required

Protocol Outline

■Storage/Stability

Standard, Biotinylated Brain Natriuretic peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

■ Antigen Information

Gene Symbol:

■ Product Specifications

Size: 1, 2, or 5 x 96-Well Strip Microplate Kit

■ Array/ELISA Format

Species Detected:

Solid Support: 96-well Microplate

Design Principle:

Quantitative/Semi-Quantitative: Quantitative

Method of Detection: Colorimetric

Compatible Sample Types: