抗FOLR1抗体(Anti-FOLR1, Mouse-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：199464
FOLR1に対する抗体(Anti-FOLR1, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Summary
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年04月29日 14時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-FOLR1, Mouse-Mono(548908) |
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Anti-Human FOLR1 MAb (Clone 548908) |
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[在庫・価格 ：2024年04月29日 14時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-FOLR1, Mouse-Mono(548908)
文献数： 4
- 商品コード：MAB5646
- メーカー：RSD
- 包装：100μg
- 価格：¥104,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human FOLR1 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF5646，スタンダードとして#5646-FR-050を用いる。 別名：Adult folate-binding protein クローン：548908 Genbank No： 2348 Protein Accession No： P15328 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Mouse | ||||
| 交差性 | Human | 適用 | ELISA,FCM,IC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
Human IFN-gamma/IL-17 Dual Color ELISpot Kit |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human FOLR1 MAb (Clone 548908)
文献数： 0
- 商品コード：MAB5646-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：Adult folate-binding protein クローン：548908 Genbank No： 2348 Protein Accession No： P15328 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,FCM,IC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human FOLR1Arg25-Met233Accession # P15328 |
| Source | Monoclonal Mouse IgG1 Clone # 548908 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 2 µg/mL | See below |
| Flow Cytometry | 0.25 µg/106 cells | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Human FOLR1 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human FOLR1 Antibody (Catalog #MAB5646 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human FOLR1 Biotinylated Antibody (Catalog #BAF5646 ) |
| ELISA Standard | Recombinant Human FOLR1 Protein, CF (Catalog #5646-FR ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Flow Cytometry |
![Flow Cytometry FOLR1 Antibody (548908) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/FOLR1_MAB5646_Flow_Cytometry_10781.jpg) click image to view larger | Detection of FOLR1 in MCF‑7 Human Cell Line by Flow Cytometry.MCF‑7 human breast cancer cell line was stained with Mouse Anti‑Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). |
| Western Blot |
![Western Blot FOLR1 Antibody (548908) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/FOLR1_MAB5646_Western_Blot_6248.jpg) click image to view larger | Detection of Human FOLR1 by Western Blot.Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FOLR1 at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. Use under non-reducing conditions only. |
| Immunocytochemistry |
![Immunocytochemistry FOLR1 Antibody (548908) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/FOLR1_MAB5646_Immunocytochemistry_6624.jpg) click image to view larger | FOLR1 in MCF-7 Human Cell Line.FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: FOLR1 | Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11). | |
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References
| Kelemen, L.E. (2006) Int. J. Cancer 119:243. | |
| Fowler, B. et al. (2001) Kidney Int. 59:S-221. | |
| Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446. | |
| Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715. | |
| Elwood, P.C. (1989) J. Biol. Chem. 264:14893. | |
| Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35. | |
| Ross, J.F. et al. (1994) Cancer 73:2432. | |
| Parker, N. et al. (2005) Anal. Biochem. 338:284. | |
| Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228. | |
| Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406. | |
| Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346. | |
| Long Name: | Folate Receptor 1 |
| Entrez Gene IDs: | 2348 (Human); 14275 (Mouse); 171049 (Rat) |
| Alternate Names: | Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18 |
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Related Research Areas
| Cancer Biomarkers | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Very Good | WB | Human | Anonymous | 05/08/2018 | Image Details |
 Western Blot: Human FOLR1 Antibody [MAB5646]. | |||
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Summary
| Application | Western Blot | |||||||||
| Sample Tested | SK-OV-3 human ovarian adenocarcinoma cell line,igrov-1,NCI-H292,HT-29 human colon adenocarcinoma cell line,CAOV3 | |||||||||
| Species | Human | |||||||||
 Enlarge | Very Good | Flow | Human | Anonymous | 04/03/2018 | Image Details |
 Flow Cytometry: Human FOLR1 Antibody [MAB5646]. |
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Summary
| Application | Flow Cytometry | |||||||||
| Sample Tested | human cancer cell line | |||||||||
| Species | Human | |||||||||
 Enlarge | Poor | WB | Human | Anonymous | 02/23/2016 | Image Details |
 Western Blot: Human FOLR1 Antibody [MAB5646]. |
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Summary
| Application | Western Blot |
| Sample Tested | Ovarian cancer tissue |
| Species | Human |
| Other Experimental Details | R&D Systems Technical Service will investigate. |
| Flow Cytometry | |
|---|---|
 | Detection of FOLR1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti‑Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). |
| Western Blot | |
 | Detection of Human FOLR1 by Western Blot. Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FOLR1 at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. Use under non-reducing conditions only. |
| Immunocytochemistry | |
 | FOLR1 in MCF-7 Human Cell Line. FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
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Related Product & Information
| Background | FOLR1 |
|---|---|
| background_content | Background: FOLR1 Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
- Promising Nanocarriers for PEDF Gene Targeting Delivery to Cervical Cancer Cells Mediated by the Over-expressing FR?
Sci Rep, 2016;6(0):32427.
Species: Human
Sample Type: Whole Cells
Application: Flow - Conditions associated with circulating tumor-associated folate receptor 1 protein in healthy men and women.
Authors: Kelemen L, Brenton J, Parkinson C, C Whitaker H, Piskorz A, Csizmadi I, Robson P
PLoS ONE, 2014;9(5):e96542.
Species: Human
Sample Type: Serum
Application: ELISA Capture - Harnessing engineered antibodies of the IgE class to combat malignancy: initial assessment of FcvarepsilonRI-mediated basophil activation by a tumour-specific IgE antibody to evaluate the risk of type I hypersensitivity.
Authors: Rudman SM, Josephs DH, Cambrook H, Karagiannis P, Gilbert AE, Dodev T, Hunt J, Koers A, Montes A, Taams L, Canevari S, Figini M, Blower PJ, Beavil AJ, Nicodemus CF, Corrigan C, Kaye SB, Nestle FO, Gould HJ, Spicer JF, Karagiannis SN
Clin. Exp. Allergy, 2011;41(10):1400-1413.
Species: Human
Sample Type: Serum
Application: ELISA Development
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