抗Human Phospho-Axl抗体(Anti-Human Phospho-Axl antibody)
掲載日情報：2018/11/26 現在Webページ番号：196483
Human Phospho-Axlに対する抗体(Anti-Human Phospho-Axl )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月20日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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文献数 | ||
|---|---|---|---|---|---|
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Anti-Human Phospho-Axl (Y779) MAb (Clone 713610) |
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2 | |||
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Anti-Human Phospho-Axl (Y779) MAb (Clone 713610) |
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0 | |||
[在庫・価格 ：2024年05月20日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human Phospho-Axl (Y779) MAb (Clone 713610)
文献数： 2
- 商品コード：MAB6965
- メーカー：RSD
- 包装：100μg
- 価格：¥99,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human Phospho-Axl (Y779) MAb (Clone 713610)
文献数： 0
- 商品コード：MAB6965-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Phosphopeptide containing the human Axl Y779 site |
| Source | Monoclonal Mouse IgG1 Clone # 713610 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human Phospho-Axl (Y779) in direct ELISAs and Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.2 µg/mL | See below |
| Simple Western | 4 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Intracellular Staining by Flow Cytometry | 2.5 µg/106 cells | See below |
| Western Blot | |
|---|---|
 | Detection of Human Phospho-Axl (Y779) by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Phospho-Axl (Y779) Monoclonal Antibody (Catalog # MAB6965) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Axl (Y779) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry | |
 | Phospho-Axl (Y779) in A172 Human Cell Line. Axl phosphorylated at Y779 and total Axl were assessed in immersion fixed A172 human glioblastoma cells incubated with (upper panel) or without (lower panel) the phosphatase inhibitor pervanadate at 100 μM for 5 minutes. Phospho-Axl was detected using Mouse Anti-Human Phospho-Axl (Y779) Monoclonal Antibody (Catalog # MAB6965) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained using DAPI (blue). Total Axl was detected using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154). Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of Phospho-Axl (Y779) in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was unstimulated (open histogram) or treated with 100 µM pervanadate for 5 minutes, then stained with Mouse Anti-Human Phospho-Axl (Y779) Monoclonal Antibody (Catalog # MAB6965, filled histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with Triton® X-100. |
| Simple Western | |
 | Detection of Human Phospho-Axl (Y779) by Simple WesternTM. Simple Western lane view shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Axl (Y779) at approximately 150 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human Phospho-Axl (Y779) Monoclonal Antibody (Catalog # MAB6965). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. |
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Related Product & Information
| Background | Axl |
|---|---|
| background_content | Background: Axl Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K‑dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions. Phosphorylation of Tyrosine 779 provides a docking site for p85 subunits of PI 3-Kinase (4). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Gilteritinib, a FLT3/AXL inhibitor, shows antileukemic activity in mouse models of FLT3 mutated acute myeloid leukemia
Authors: M Mori, N Kaneko, Y Ueno, M Yamada, R Tanaka, R Saito, I Shimada, K Mori, S Kuromitsu
Invest New Drugs, 2017;0(0):.
Species: Human
Sample Type: Cell Lysates
Application: WB
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