抗Human/Mouse/Rat Cyclophilin B抗体(Anti-Human/Mouse/Rat Cyclophilin B antibody)
掲載日情報：2018/11/26 現在Webページ番号：196268
Human/Mouse/Rat Cyclophilin Bに対する抗体(Anti-Human/Mouse/Rat Cyclophilin B )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年04月29日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human/Mouse/Rat Cyclophilin B MAb (Clone 549205) |
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Anti-Human/Mouse/Rat Cyclophilin B MAb (Clone 549205) |
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[在庫・価格 ：2024年04月29日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human/Mouse/Rat Cyclophilin B MAb (Clone 549205)
文献数： 0
- 商品コード：MAB5410
- メーカー：RSD
- 包装：100μg
- 価格：¥100,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：Cyclophilin B クローン：549205 Genbank No： 5479 Protein Accession No： P23284 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | 法規備考 | ||||||
| 抗原種 | Human/Mouse/Rat | 免疫動物 | Mouse | ||||
| 交差性 | Human | 適用 | IC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
Human IFN-gamma/IL-17 Dual Color ELISpot Kit |
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| 関連記事 | |||||||
Anti-Human/Mouse/Rat Cyclophilin B MAb (Clone 549205)
文献数： 0
- 商品コード：MAB5410-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human, Mouse, Rat |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human Cyclophilin BAsp34-Glu216Accession # P23284 |
| Source | Monoclonal Mouse IgG2A Clone # 549205 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human, mouse, and rat Cyclophilin B in Western blots. In Western blots, no cross-react with recombinant human Cyclophilin A is observed. Detection of mouse and rat Cyclophilin B has not been tested in Immunocytochemistry. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Knockout Validated | Cyclophilin B is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Cyclophilin B knockout HeLa cell line. | |
| Western Blot | |
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 | Detection of Human/Mouse/Rat Cyclophilin B by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and NRK rat normal kidney cell. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Cyclophilin A and Cyclophilin B (5 ng/lane) were included. A specific band for Cyclophilin B was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
| Immunocytochemistry | |
 | Cyclophilin B in HeLa Human Cell Line. Cyclophilin B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Knockout Validated | |
 | Western Blot Shows Human Cyclophilin B Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Cyclophilin B Monoclonal Antibody (Catalog # MAB5410) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cyclophilin B at approximately 25 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Related Product & Information
| Entrez Gene IDs | 5479 (Human); 19035 (Mouse); 64367 (Rat) |
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| Background | Cyclophilin B |
| background_content | Background: Cyclophilin B Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27‑34), a PPIase domain (aa 47‑204) and a C-terminal ER retention motif (aa 213‑216). Over aa 34‑216, the human and mouse sequences are 95% aa identical. |
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