抗Human PLZF Allophycocyanin抗体(Anti-Human PLZF Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	E. coli-derived recombinant human PLZFMet1-Gln254Accession # Q05516
Source	Monoclonal Mouse IgG2A Clone # 6318100
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human PLZF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZBTB38, rhZNF24, rhZNF143, rhZNF206, rhZNF281, or rhZNF423 is observed.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of PLZF in HL‑60 Human Cell Line by Flow Cytometry. HL‑60 human acute promyelocytic leukemia cell line was stained with Mouse Anti-Human PLZF APC‑conjugated Monoclonal Antibody (Catalog # IC2944A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Long Name	Promyelocytic Leukemia Zinc Finger
Background	PLZF
background_content	Background: PLZF Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.

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Citations

1. MAIT cells are reduced in frequency and functionally impaired in human T lymphotropic virus type 1 infection: Potential clinical implications
   Authors: D Paquin-Pro, BC Greenspun, EA Costa, AC Segurado, EG Kallas, DF Nixon, FE Leal
   PLoS ONE, 2017;12(4):e0175345.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Acquisition of innate-like microbial reactivity in mucosal tissues during human fetal MAIT-cell development.
   Authors: Leeansyah E, Loh L, Nixon D, Sandberg J
   Nat Commun, 2014;5(0):3143.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: Flow


3. TLR-induced cytokines promote effective proinflammatory natural Th17 cell responses.
   Authors: Massot B, Michel M, Diem S, Ohnmacht C, Latour S, Dy M, Eberl G, Leite-de-Moraes M
   J Immunol, 2014;192(12):5635-42.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Human iNKT and MAIT cells exhibit a PLZF-dependent proapoptotic propensity that is counterbalanced by XIAP.
   Authors: Gerart S, Siberil S, Martin E, Lenoir C, Aguilar C, Picard C, Lantz O, Fischer A, Latour S
   Blood, 2013;121(4):614-23.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Activation, exhaustion, and persistent decline of the antimicrobial MR1-restricted MAIT-cell population in chronic HIV-1 infection.
   Authors: Leeansyah E, Ganesh A, Quigley M, Sonnerborg A, Andersson J, Hunt P, Somsouk M, Deeks S, Martin J, Moll M, Shacklett B, Sandberg J
   Blood, 2013;121(7):1124-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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