抗Human IL-1 beta/IL-1F2 PerCP抗体(Anti-Human IL-1 beta/IL-1F2 PerCP antibody)

掲載日情報:2018/11/26 現在Webページ番号:195828

Human IL-1 beta/IL-1F2 PerCPに対する抗体(Anti-Human IL-1 beta/IL-1F2 PerCP )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2024年04月29日 11時15分現在]

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Anti-Human IL-1 beta/IL-1F2 PerCP MAb (Clone 8516)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
別名:catabolin
クローン:8516
Genbank No: 3553
Protein Accession No: P01584
法規制等
保存条件 法規備考
抗原種 Human 免疫動物 Mouse クラス IgG 標識 PerCP
交差性 Human 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

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[在庫・価格 :2024年04月29日 11時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Human IL-1 beta/IL-1F2 PerCP MAb (Clone 8516)

文献数: 0

説明文 別名:catabolin
クローン:8516
Genbank No: 3553
Protein Accession No: P01584
法規制等
保存条件 法規備考
抗原種 Human 免疫動物 Mouse
交差性 Human 適用 FCM
標識 PerCP 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 Mouse IL-2/IL-10 Dual Color ELISpot Kit
M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
R&D Systems社 IL-1β関連製品
関連記事



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Product Details

Species ReactivityHuman
LabelPerCP (Peridinin-chlorophyll Protein Complex)
ImmunogenE. coli-derived recombinant human IL-1 beta /IL-1F2aa 117-269Accession # P01584
SourceMonoclonal Mouse IgG1 Clone # 8516
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL‑1 beta and rpIL‑1 beta and no cross-reactivity with rrIL‑1 beta, rmIL‑1 alpha, rhIL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.


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Applications and Data

 Recommended
Concentration
Sample
Intracellular Staining by Flow Cytometry10 µL/106 cellsSee below


Intracellular Staining by Flow Cytometry
Detection of IL‑1 beta /IL‑1F2 in LPS-treated Peripheral Blood Mononuclear Cell Monocytes by Flow Cytometry.
Peripheral blood mononuclear cell monocytes, resting (open histogram) or treated with LPS (filled histogram), were stained with Mouse Anti-Human IL‑1 beta /IL‑1F2 PerCP‑conjugated Monoclonal Antibody (Catalog # IC201C) or isotype control antibody (Catalog # IC002C). View our protocol for Staining Intracellular Molecules.


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Related Product & Information

BackgroundIL-1 beta/IL-1F2
background_contentBackground:
IL-1 beta/IL-1F2
IL-1 is a name that defines two 17 kDa pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), both of which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related single-chain polypeptides that share approximately 21% amino acid (aa) identity in human. Although IL-1 ( alpha and beta ) references two distinct molecules, the term IL-1 is also generally applied to an eleven-member protein family that exhibits an Ala-xxx-Asp motif in its primary structure (1,3). IL-1 alpha and beta are not redundant. IL-1 alpha is constitutively expressed, contains an NLS, is active as a proform, and may present as a membrane-bound form due to myristoylation and glycosylation (4). Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, and they bind to the same cell surface receptor, the functional 80 kDa IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra, a third member of the IL-1 subfamily that also includes IL-33, functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (2,3). The human IL-1 beta cDNA encodes a 269 aa precursor that contains a 116 aa propeptide that is cleaved intracellularly by the inflammasome-associated cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate an active 153 aa cytokine (3,5,6). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% aa sequence identity with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.


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