抗IgG 二次抗体(Anti-IgG, Rat, Goat-Poly, HRP antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Rat
Label	HRP (Horseradish Peroxidase)
Immunogen	Rat IgG
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects rat IgG1, IgG2A, IgG2B heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human IgG and rabbit IgG is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	Use at a 1:1000 dilution.
Simple Western	Use at a 1:50 dilution.

Western Blot
	Detection of Rat IgGPrimary Antibody by Western Blot Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a MonoclonalAntibody (Catalog # MAB645)using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody(Catalog # HAF005).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.
Simple Western
	Detection of Rat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of RatAnti-Mouse S100A9 Monoclonal Antibody (Catalog #MAB2065)using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions andusing the 12-230 kDa separation system.

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Related Product & Information

Long Name	Immunoglobulin G
Background	IgG
background_content	Background: IgG R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

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Citations

1. Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3
   Authors: L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner
   PLoS ONE, 2018;13(5):e0195427.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


2. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
   Nat. Med, 2016;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. The Processed Amino-Terminal Fragment of Human TLR7 Acts as a Chaperone To Direct Human TLR7 into Endosomes.
   Authors: Hipp M, Shepherd D, Booth S, Waithe D, Reis E Sousa C, Cerundolo V
   J Immunol, 2015;194(11):5417-25.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells.
   Authors: Khan A, Amu S, Saunders S, Hams E, Blackshields G, Leonard M, Weaver C, Sparwasser T, Sheils O, Fallon P
   Eur J Immunol, 2015;45(6):1842-54.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: Neut


5. Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
   Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
   Kidney Int, 2014;85(6):1340-50.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


6. Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
   Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
   Sci Rep, 2014;4(0):6315.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
   Authors: Suryawanshi A, Mulik S, Sharma S
   J. Immunol., 2011;186(6):3653-65.
   Species: N/A
   Sample Type: N/A
   Application: WB


8. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
   Authors: Reddy PB, Sehrawat S, Suryawanshi A
   J. Immunol., 2011;187(11):5745-55.
   Species: N/A
   Sample Type: N/A
   Application: WB

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