抗Mouse EDG-1/S1P1 Allophycocyanin抗体(Anti-Mouse EDG-1/S1P1 Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Allophycocyanin
Immunogen	Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)Accession # O08530
Source	Monoclonal Rat IgG2A Clone # 713412
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and eGFP was stained with either (A) Rat Anti-Mouse S1P1/EDG‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB7089A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection of S1P1/EDG‑1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB7089A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Long Name	Endothelial Differentiation Gene 1
Background	S1P1/EDG-1
background_content	Background: S1P1/EDG-1 S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

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Citations

1. Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1
   Authors: R Mashud, A Nomachi, A Hayakawa, K Kubouchi, S Danno, T Hirata, K Matsuo, T Nakayama, R Satoh, R Sugiura, M Abe, K Sakimura, S Wakana, H Ohsaki, S Kamoshida, H Mukai
   Sci Rep, 2017;7(1):7663.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. IL4RA on lymphatic endothelial cells promotes T cell egress during sclerodermatous graft versus host disease
   JCI Insight, 2016;1(12):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Targeting of Ly9 (CD229) Disrupts Marginal Zone and B1 B Cell Homeostasis and Antibody Responses.
   Authors: Cuenca M, Romero X, Sintes J, Terhorst C, Engel P
   J Immunol, 2016;196(2):726-37.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Intravenous gammaglobulin inhibits encephalitogenic potential of pathogenic T cells and interferes with their trafficking to the central nervous system, implicating sphingosine-1 phosphate receptor 1-mammalian target of rapamycin axis.
   Authors: Othy S, Hegde P, Topcu S, Sharma M, Maddur M, Lacroix-Desmazes S, Bayry J, Kaveri S
   J Immunol, 2013;190(9):4535-41.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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