抗Mouse MMR/CD206 Phycoerythrin抗体(Anti-Mouse MMR/CD206 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206Leu19-Ala1388Accession # Q2HZ94
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human MMR is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of MMR/CD206 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse MMR/CD206 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2535P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

MMR/CD206

background_content

Background: MMR/CD206 The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

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Citations

1. Deletion of lactate dehydrogenase-A in myeloid cells triggers antitumor immunity
   Authors: P Seth, E Csizmadia, A Hedblom, M Vuerich, H Xie, M Li, MS Longhi, B Wegiel
   Cancer Res., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Differential Immunometabolic Phenotype in Th1 and Th2 Dominant Mouse Strains in Response to High-Fat Feeding.
   Authors: Jovicic N, Jeftic I, Jovanovic I, Radosavljevic G, Arsenijevic N, Lukic M, Pejnovic N
   PLoS ONE, 2015;10(7):e0134089.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Obesity alters adipose tissue macrophage iron content and tissue iron distribution.
   Authors: Orr J, Kennedy A, Anderson-Baucum E, Webb C, Fordahl S, Erikson K, Zhang Y, Etzerodt A, Moestrup S, Hasty A
   Diabetes, 2014;63(2):421-32.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Alveolar epithelial cells are critical in protection of the respiratory tract by secretion of factors able to modulate the activity of pulmonary macrophages and directly control bacterial growth.
   Authors: Chuquimia O, Petursdottir D, Periolo N, Fernandez C
   Infect Immun, 2013;81(1):381-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype.
   Authors: Ylostalo J, Bartosh T, Coble K, Prockop D
   Stem Cells, 2012;30(10):2283-96.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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