抗TIM-3抗体(Anti-TIM-3, Rat-Mono, PerCP antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	PerCP (Peridinin-chlorophyll Protein Complex)
Immunogen	Mouse myeloma cell line NS0-derived recombinant human TIM‑3Ser22-Arg200Accession # Q8TDQ0.2
Source	Monoclonal Rat IgG2A Clone # 344823
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM‑3 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2365C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

TIM-3

background_content

Background: TIM-3 TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8+ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

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Citations

1. Antiparasitic Treatment Induces an Improved CD8(+) T Cell Response in Chronic Chagasic Patients
   Authors: J Mateus, E Pérez-Antó, P Lasso, A Egui, N Roa, B Carrilero, JM González, MC Thomas, CJ Puerta, MC López, A Cuéllar
   J. Immunol, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Inhibitory Receptor Expression on CD8+ T Cells Is Linked to Functional Responses against Trypanosoma cruzi Antigens in Chronic Chagasic Patients.
   Authors: Lasso P, Mateus J, Pavia P, Rosas F, Roa N, Thomas M, Lopez M, Gonzalez J, Puerta C, Cuellar A
   J Immunol, 2015;195(8):3748-58.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. CD127 expression, exhaustion status and antigen specific proliferation predict sustained virologic response to IFN in HCV/HIV co-infected individuals.
   Authors: Kared H, Saeed S, Klein M, Shoukry N
   PLoS ONE, 2014;9(7):e101441.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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