抗Human IL-17 RC Allophycocyanin抗体(Anti-Human IL-17 RC Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human IL‑17 RCLeu21-Ala454Accession # NP_116121
Source	Monoclonal Mouse IgG2B Clone # 309822
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-17 RC in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑17 R, rhIL-17 BR, rhIL-17 RD, or recombinant mouse IL-17 RC is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of IL‑17 RC in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL‑17 RC APC‑conjugated Monoclonal Antibody (Catalog # FAB22691A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

IL-17 RC

background_content

Background: IL-17 RC IL-17 receptor C (IL-17 RC; also known as IL-17 RL and IL-17 Rhom) is an 85‑110 kDa member of the IL-17 receptor family. This is one of five families that currently comprise the cytokine receptor superfamily (1, 2, 3, 4, 5, 6). At this time, there are five members within the IL-17 receptor family, and these are termed IL-17 RA, B, C, D and E. Not all receptors appear to bind known members of the IL-17 cytokine family. To date, IL-17 RA is reported to bind IL-17(A), while IL-17 RB is reported to bind IL-17B and IL-17E (2, 4). Human IL-17 RC is a type I transmembrane glycoprotein that is expressed on a variety of nonhematopoietic cell types. These include endothelial cells (6, 7), chondrocytes and osteoblasts (8), breast and prostatic epithelium (6), and fibroblasts, plus renal tubular epithelium and skeletal muscle cells (8, 9). Full-length IL-17 RC is synthesized as a 791 amino acid (aa) precursor (10, 11). It contains a 20 aa signal sequence, a 518 aa extracellular domain (ECD) (aa 21‑538), a 21 aa transmembrane segment, and a 232 aa cytoplasmic region. Although IL-17 RA has two fibrinogen-like regions in its ECD that contribute to its function, no such architecture has been identified in the ECD of IL-17 RC (12). Based on studies looking at exon deletions, a key ligand-binding site would appear to exist over aa 425‑441 (13). The gene for human IL-17 RC contains 19 exons. It is estimated that there are over 90 alternative splice forms, with transmembrane-containing isoforms predominating (6, 14). The full-length isoform is estimated to occur approximately 10% of the time, while the three most common isoforms, as a group, occur about 50% of the time. Based on limited information, alternative splicing appears to regulate ligand specificity (13). R&D Systems IL-17 RC corresponds to IL-17 RC isoform # 3, which shows deletions of aa 36‑106 and 264‑278 relative to the full-length form (10). Over the ECD, IL-17 RC isoform #3 is 68% aa identical to mouse IL-17 RC ECD. IL-17 RC is the cognate receptor for IL-17F, and binds IL-17A with similar affinity (13). With IL-17 RA, it forms a definitive receptor for both IL-17A and IL-17F. The stoichiometry is unclear; it may form a heterodimer with IL-17 RA, or a heterotrimer with a preexisting IL-17 RA homodimer (4, 9, 13, 15). The heteromeric nature of the receptor may be important given that the predominant form of the IL-17 cytokine is now considered to be an IL-17A:IL-17F heterodimer (4).

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Citations

1. Overexpression of IL-17RC associated with ocular sarcoidosis.
   Authors: Wu W, Jin M, Wang Y, Liu B, Shen D, Chen P, Hannes S, Li Z, Hirani S, Jawad S, Sen H, Chan C, Nussenblatt R, Wei L
   J Transl Med, 2014;12(0):152.
   Species: Human
   Sample Type: Whole Blood
   Application: Flow


2. TH17 cytokines induce human airway smooth muscle cell migration.
   Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
   J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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