抗Human ALDH3A1抗体(Anti-Human ALDH3A1 antibody)
掲載日情報：2018/11/26 現在Webページ番号：194934
Human ALDH3A1に対する抗体(Anti-Human ALDH3A1 )です。
※ 本製品は研究用です。研究用以外には使用できません。
カートに商品を
追加しました。
追加しました。
価格
[在庫・価格 ：2024年05月20日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
|
文献数 | ||
|---|---|---|---|---|---|
|
Anti-Human ALDH3A1 Affinity Purified PAb |
|
0 | |||
|
Anti-Human ALDH3A1 Affinity Purified PAb |
|
0 | |||
[在庫・価格 ：2024年05月20日 20時55分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human ALDH3A1 Affinity Purified PAb
文献数： 0
- 商品コード：AF6705
- メーカー：RSD
- 包装：100μg
- 価格：¥100,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human ALDH3A1 Affinity Purified PAb
文献数： 0
- 商品コード：AF6705-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
カートに商品を
追加しました。
追加しました。
Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 Ser2-His453Accession # P30838 |
| Source | Polyclonal Sheep IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 in direct ELISAs and Western blots. |
カートに商品を
追加しました。
追加しました。
Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.25 µg/mL | See below |
| Immunohistochemistry | 3-15 µg/mL | See below |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Western Blot | |
|---|---|
 | Detection of Human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 by Western Blot. Western blot shows lysates of human stomach tissue, human lung tissue, human liver tissue, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human Aldehyde Dehydrogenase3‑A1/ALDH3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6705) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldehyde Dehydrogenase 3‑A1/ALDH3A1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Immunocytochemistry | |
 | Aldehyde Dehydrogenase 3‑A1/ALDH3A1 in A549 Human Cell Line. Aldehyde Dehydrogenase 3‑A1/ALDH3A1 was detected in immersion fixed A549 human lung carcinoma cell line using Sheep Anti-Human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6705) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunohistochemistry | |
 | Aldehyde Dehydrogenase 3‑A1/ALDH3A1 in Human Normal Lung and Lung Cancer Tissue. Aldehyde Dehydrogenase 3‑A1/ALDH3A1 was detected in immersion fixed paraffin-embedded sections of human normal lung and lung cancer tissue using Sheep Anti-Human Aldehyde Dehydrogenase 3‑A1/ALDH3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6705) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
カートに商品を
追加しました。
追加しました。
Related Product & Information
| Entrez Gene IDs | 218 (Human); 11670 (Mouse); 25375 (Rat) |
|---|---|
| Background | Aldehyde Dehydrogenase 3-A1/ALDH3A1 |
| background_content | Background: Aldehyde Dehydrogenase 3-A1/ALDH3A1 ALDH3A1 (Aldehyde dehydrogenase 3; also aldehyde dehydrogenase, dimeric NADP-preferring) is a 51-55 kDa, nuclear and cytosolic member of the ALDH3 family, ALDH superfamily of enzymes. It has widespread expression, being found in pancreatic acinar cells, corneal epithelium and stromal keratocytes, stem cells, and likely the epithelium of the jejunum. ALDH3A1 has many activities, including the detoxification of lipid peroxidation-derived medium chain (>5 carbon) aldehydes, the absorption of UV light in the cornea, acting as an antioxidant through the production of NADPH, and the extension of the cell cycle in expressing cells by reducing cyclin-dependent kinase activity. Notably, the critically important absorption of UVB light by ALDH3A1 in the cornea induces its aggregation and renders the molecule inactive as an enzyme. Human ALDH3A1 is 453 amino acids (aa) in length. It contains four acetylated lysines and a general catalytic region between aa 5-447. There are potentially six splice variants, one of which shows a 130 aa substitution for aa 1-247. Over aa 2-453, human ALDH3A1 shares 80% and 61% aa sequence identity with mouse ALDH3A1 asnd human ALDH3A2, respectively. |
カートに商品を
追加しました。
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。